Recombinant Human CD14 Protein (Active) is a Human Full Length protein, in the 20 to 352 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
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Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
HPLC, MS, SDS-PAGE
- Biologically active
Yes
Amino acid sequence
T T P E P C E L D D E D F R C V C N F S E P Q P D W S E A F Q C V S A V E V E I H A G G L N L E P F L K R V D A D A D P R Q Y A D T V K A L R V R R L T V G A A Q V P A Q L L V G A L R V L A Y S R L K E L T L E D L K I T G T M P P L P L E A T G L A L S S L R L R N V S W A T G R S W L A E L Q Q W L K P G L K V L S I A Q A H S P A F S C E Q V R A F P A L T S L D L S D N P G L G E R G L M A A L C P H K F P A I Q N L A L R N T G M E T P T G V C A A L A A A G V Q P H S L D L S H N S L R A T V N P S A P R C M W S S A L N S L N L S F A G L E Q V P K G L P A K L R V L D L S C N R L N R A P Q P D E L P E V D N L T L D G N P F L V P G T A L P H E G S M N S G V V P A C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655, PubMed:22265692). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
Alternative names
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14
Recommended products
Recombinant Human CD14 Protein (Active) is a Human Full Length protein, in the 20 to 352 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
HPLC, MS, SDS-PAGE
- Biologically active
Yes
- Biological activity
Fully biologically active determined by its ability to enhance LPS-induced IL-8 secretion in THP-1 human acute monocytic leukemia cells.
ED50 is ≤ 49.2 ng/ml, corresponding to a specific activity of 2.03 x 104 units/mg.
- Accession
- P08571-1
- Animal free
Yes
- Species
Human
- Reconstitution
Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
T T P E P C E L D D E D F R C V C N F S E P Q P D W S E A F Q C V S A V E V E I H A G G L N L E P F L K R V D A D A D P R Q Y A D T V K A L R V R R L T V G A A Q V P A Q L L V G A L R V L A Y S R L K E L T L E D L K I T G T M P P L P L E A T G L A L S S L R L R N V S W A T G R S W L A E L Q Q W L K P G L K V L S I A Q A H S P A F S C E Q V R A F P A L T S L D L S D N P G L G E R G L M A A L C P H K F P A I Q N L A L R N T G M E T P T G V C A A L A A A G V Q P H S L D L S H N S L R A T V N P S A P R C M W S S A L N S L N L S F A G L E Q V P K G L P A K L R V L D L S C N R L N R A P Q P D E L P E V D N L T L D G N P F L V P G T A L P H E G S M N S G V V P A C
- Accession
- Protein length
Full Length
- Predicted molecular weight
35.83 kDa
- Actual molecular weight
35.83 kDa
- Amino acids
20 to 352
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655, PubMed:22265692). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
- Post-translational modifications
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
- Biological function summary
This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.
- Pathways
CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.
- Associated diseases and disorders
Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
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4 product images
Mass Spectrometry - Recombinant Human CD14 Protein (Active) (ab290112)
Mass determination by ESI-TOF.
Predicted MW is 35830.06 Da (+/-10 Da by ESI-TOF). Observed MW is 35834.28 Da.SDS-PAGE - Recombinant Human CD14 Protein (Active) (ab290112)
SDS-PAGE analysis of ab290112
Biological Activity - Recombinant Human CD14 Protein (Active) (ab290112)
Fully biologically active determined by its ability to enhance LPS-induced IL-8 secretion in THP-1 human acute monocytic leukemia cells.
ED50 is ≤ 49.2 ng/ml, corresponding to a specific activity of 2.03 x 10^4 units/mg.
HPLC - Recombinant Human CD14 Protein (Active) (ab290112)
HPLC analysis of ab290112
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