Recombinant Human CD163 protein (His tag) is a Human Fragment protein, in the 42 to 1045 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S S L G G T D K E L R L V D G E N K C S G R V E V K V Q E E W G T V C N N G W S M E A V S V I C N Q L G C P T A I K A P G W A N S S A G S G R I W M D H V S C R G N E S A L W D C K H D G W G K H S N C T H Q Q D A G V T C S D G S N L E M R L T R G G N M C S G R I E I K F Q G R W G T V C D D N F N I D H A S V I C R Q L E C G S A V S F S G S S N F G E G S G P I W F D D L I C N G N E S A L W N C K H Q G W G K H N C D H A E D A G V I C S K G A D L S L R L V D G V T E C S G R L E V R F Q G E W G T I C D D G W D S Y D A A V A C K Q L G C P T A V T A I G R V N A S K G F G H I W L D S V S C Q G H E P A I W Q C K H H E W G K H Y C N H N E D A G V T C S D G S D L E L R L R G G G S R C A G T V E V E I Q R L L G K V C D R G W G L K E A D V V C R Q L G C G S A L K T S Y Q V Y S K I Q A T N T W L F L S S C N G N E T S L W D C K N W Q W G G L T C D H Y E E A K I T C S A H R E P R L V G G D I P C S G R V E V K H G D T W G S I C D S D F S L E A A S V L C R E L Q C G T V V S I L G G A H F G E G N G Q I W A E E F Q C E G H E S H L S L C P V A P R P E G T C S H S R D V G V V C S R Y T E I R L V N G K T P C E G R V E L K T L G A W G S L C N S H W D I E D A H V L C Q Q L K C G V A L S T P G G A R F G K G N G Q I W R H M F H C T G T E Q H M G D C P V T A L G A S L C P S E Q V A S V I C S G N Q S Q T L S S C N S S S L G P T R P T I P E E S A V A C I E S G Q L R L V N G G G R C A G R V E I Y H E G S W G T I C D D S W D L S D A H V V C R Q L G C G E A I N A T G S A H F G E G T G P I W L D E M K C N G K E S R I W Q C H S H G W G Q Q N C R H K E D A G V I C S E F M S L R L T S E A S R E A C A G R L E V F Y N G A W G T V G K S S M S E T T V G V V C R Q L G C A D K G K I N P A S L D K A M S I P M W V D N V Q C P K G P D T L W Q C P S S P W E K R L A S P S E E T W I T C D N K I R L Q E G P T S C S G R V E I W H G G S W G T V C D D S W D L D D A Q V V C Q Q L G C G P A L K A F K E A E F G Q G T G P I W L N E V K C K G N E S S L W D C P A R R W G H S E C G H K E D A A V N C T D I S V Q K T P Q K A T T G R S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Alternative names
CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor
Recommended products
Recombinant Human CD163 protein (His tag) is a Human Fragment protein, in the 42 to 1045 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q86VB7-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 5% Trehalose
Sequence info
Amino acid sequence
- S S L G G T D K E L R L V D G E N K C S G R V E V K V Q E E W G T V C N N G W S M E A V S V I C N Q L G C P T A I K A P G W A N S S A G S G R I W M D H V S C R G N E S A L W D C K H D G W G K H S N C T H Q Q D A G V T C S D G S N L E M R L T R G G N M C S G R I E I K F Q G R W G T V C D D N F N I D H A S V I C R Q L E C G S A V S F S G S S N F G E G S G P I W F D D L I C N G N E S A L W N C K H Q G W G K H N C D H A E D A G V I C S K G A D L S L R L V D G V T E C S G R L E V R F Q G E W G T I C D D G W D S Y D A A V A C K Q L G C P T A V T A I G R V N A S K G F G H I W L D S V S C Q G H E P A I W Q C K H H E W G K H Y C N H N E D A G V T C S D G S D L E L R L R G G G S R C A G T V E V E I Q R L L G K V C D R G W G L K E A D V V C R Q L G C G S A L K T S Y Q V Y S K I Q A T N T W L F L S S C N G N E T S L W D C K N W Q W G G L T C D H Y E E A K I T C S A H R E P R L V G G D I P C S G R V E V K H G D T W G S I C D S D F S L E A A S V L C R E L Q C G T V V S I L G G A H F G E G N G Q I W A E E F Q C E G H E S H L S L C P V A P R P E G T C S H S R D V G V V C S R Y T E I R L V N G K T P C E G R V E L K T L G A W G S L C N S H W D I E D A H V L C Q Q L K C G V A L S T P G G A R F G K G N G Q I W R H M F H C T G T E Q H M G D C P V T A L G A S L C P S E Q V A S V I C S G N Q S Q T L S S C N S S S L G P T R P T I P E E S A V A C I E S G Q L R L V N G G G R C A G R V E I Y H E G S W G T I C D D S W D L S D A H V V C R Q L G C G E A I N A T G S A H F G E G T G P I W L D E M K C N G K E S R I W Q C H S H G W G Q Q N C R H K E D A G V I C S E F M S L R L T S E A S R E A C A G R L E V F Y N G A W G T V G K S S M S E T T V G V V C R Q L G C A D K G K I N P A S L D K A M S I P M W V D N V Q C P K G P D T L W Q C P S S P W E K R L A S P S E E T W I T C D N K I R L Q E G P T S C S G R V E I W H G G S W G T V C D D S W D L D D A Q V V C Q Q L G C G P A L K A F K E A E F G Q G T G P I W L N E V K C K G N E S S L W D C P A R R W G H S E C G H K E D A A V N C T D I S V Q K T P Q K A T T G R S
- Accession
- Q86VB7
- Protein length
- Fragment
- Predicted molecular weight
- 110.4 kDa
- Amino acids
- 42 to 1045
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
- Post-translational modifications
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
- Biological function summary
CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.
- Pathways
CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.
- Associated diseases and disorders
CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.
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1 product image
SDS-PAGE - Recombinant Human CD163 protein (His tag) (ab219873)
SDS PAGE analysis of ab219873. DTT-reduced protein migrates as 135-140 kDa due to glycosylation.
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