Recombinant human CD167a/DDR1 (mutated S496A) protein is a Human Fragment protein, in the 444 to 876 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >75% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
R R L L S K A E R R V L E E E L T V H L S V P G D T I L I N N R P G P R E P P P Y Q E P R P R G N P P H A A P C V P N G S A Y S G D Y M E P E K P G A P L L P P P P Q N S V P H Y A E A D I V T L Q G V T G G N T Y A V P A L P P G A V G D G P P R V D F P R S R L R F K E K L G E G Q F G E V H L C E V D S P Q D L V S L D F P L N V R K G H P L L V A V K I L R P D A T K N A R N D F L K E V K I M S R L K D P N I I R L L G V C V Q D D P L C M I T D Y M E N G D L N Q F L S A H Q L E D K A A E G A P G D G Q A A Q G P T I S Y P M L L H V A A Q I A S G M R Y L A T L N F V H R D L A T R N C L V G E N F T I K I A D F G M S R N L Y A G D Y Y R V Q G R A V L P I R W M A W E C I L M G K F T T A S D V W A F G V T L W E V L M L C R A Q P F G Q L T D E Q V I E N A G E F F R D Q G R Q V Y L S R P P A C P Q G L Y E L M L R C W S R E S E Q R P P F S Q L H R F L A E D A L N T V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
Alternative names
CD167a, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, DDR1, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2, Mammary carcinoma kinase 10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK, MCK-10
Recommended products
Recombinant human CD167a/DDR1 (mutated S496A) protein is a Human Fragment protein, in the 444 to 876 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >75% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab204092 was determined to be 1.8 nmol/min/mg.
- Accession
- Q08345-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- R R L L S K A E R R V L E E E L T V H L S V P G D T I L I N N R P G P R E P P P Y Q E P R P R G N P P H A A P C V P N G S A Y S G D Y M E P E K P G A P L L P P P P Q N S V P H Y A E A D I V T L Q G V T G G N T Y A V P A L P P G A V G D G P P R V D F P R S R L R F K E K L G E G Q F G E V H L C E V D S P Q D L V S L D F P L N V R K G H P L L V A V K I L R P D A T K N A R N D F L K E V K I M S R L K D P N I I R L L G V C V Q D D P L C M I T D Y M E N G D L N Q F L S A H Q L E D K A A E G A P G D G Q A A Q G P T I S Y P M L L H V A A Q I A S G M R Y L A T L N F V H R D L A T R N C L V G E N F T I K I A D F G M S R N L Y A G D Y Y R V Q G R A V L P I R W M A W E C I L M G K F T T A S D V W A F G V T L W E V L M L C R A Q P F G Q L T D E Q V I E N A G E F F R D Q G R Q V Y L S R P P A C P Q G L Y E L M L R C W S R E S E Q R P P F S Q L H R F L A E D A L N T V
- Accession
- Q08345
- Protein length
- Fragment
- Predicted molecular weight
- 77 kDa
- Amino acids
- 444 to 876
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
- Post-translational modifications
Autophosphorylated in response to fibrillar collagen binding.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as MCK10
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD167a also known as DDR1 (Discoidin Domain Receptor 1) is a receptor tyrosine kinase with a molecular mass of approximately 101 kDa. This protein is expressed widely in various tissues including the thyroid lung kidney and mammary gland. It becomes activated upon collagen binding leading to the regulation of cell adhesion proliferation and extracellular matrix remodeling. The DDR1 protein plays a mechanistic role in responding to unique collagen-rich environments within the body.
- Biological function summary
CD167a/DDR1 modulates interactions between cells and their surrounding matrix. It is involved in a complex with collagen mediating signal transduction processes. This interaction is important for cellular biophysical responses driving processes like cell migration and differentiation. In marmoset thyroid tissue DDR1 helps maintain structural integrity and regulates cellular activities important for normal thyroid function.
- Pathways
CD167a/DDR1 participates in key signaling pathways including those related to cell adhesion and extracellular matrix remodeling. Specifically it operates within the MAPK/ERK pathway influencing cell cycle dynamics and responses to stress signals. DDR1 also interacts with proteins like SHC and Ras enabling communication between the cell surface and intracellular targets necessary for growth and adaptation.
- Associated diseases and disorders
Mutations or irregular expression of CD167a/DDR1 have implications in cancer and fibrotic diseases. Overexpression or aberration of DDR1 can contribute to tumorigenesis facilitating tumor cell invasion and metastasis. In fibrotic conditions altered DDR1 activity can disrupt normal matrix remodeling linking it to proteins such as collagen I and III which play major roles in these disorders. Understanding DDR1's role offers potential for therapeutic interventions in targeting related pathological conditions.
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2 product images
Functional Studies - Recombinant human CD167a/DDR1 (mutated S496A) protein (ab204092)
Kinase assay showing the specific activity of ab204092 as 1.8 nmol/min/mg.
SDS-PAGE - Recombinant human CD167a/DDR1 (mutated S496A) protein (ab204092)
SDS-PAGE analysis of ab204092.
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