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AB101591

Recombinant human CD167a/DDR1 protein

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Recombinant human CD167a/DDR1 protein is a Human Fragment protein, in the 444 to 876 aa range, expressed in Baculovirus, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

CD167a, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, DDR1, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2, Mammary carcinoma kinase 10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK, MCK-10

4 Images
Functional Studies - Recombinant human CD167a/DDR1 protein (AB101591)
  • FuncS

Unknown

Functional Studies - Recombinant human CD167a/DDR1 protein (AB101591)

Specific activity of ab101591

Functional Studies - Recombinant human CD167a/DDR1 protein (AB101591)
  • FuncS

Unknown

Functional Studies - Recombinant human CD167a/DDR1 protein (AB101591)

The specific activity of MCK10/NEP (ab101591) was determined to be 6 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human CD167a/DDR1 protein (AB101591)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CD167a/DDR1 protein (AB101591)

SDS-PAGE analysis of ab101591

SDS-PAGE - Recombinant human CD167a/DDR1 protein (AB101591)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CD167a/DDR1 protein (AB101591)

SDS PAGE analysis of ab101591

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus

Tags

Tag free

Applications

FuncS, WB, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of CD167a/DDR1 is determined to be 5 nmol/min/mg

Accession

Q08345

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204867 (IRS1 peptide) can be utilized as a substrate for assessing kinase activity

This product was previously labelled as MCK10

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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"77 kDa","actualMolecularWeight":null,"aminoAcidEnd":876,"aminoAcidStart":444,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q08345","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD167a also known as DDR1 (Discoidin Domain Receptor 1) is a receptor tyrosine kinase with a molecular mass of approximately 101 kDa. This protein is expressed widely in various tissues including the thyroid lung kidney and mammary gland. It becomes activated upon collagen binding leading to the regulation of cell adhesion proliferation and extracellular matrix remodeling. The DDR1 protein plays a mechanistic role in responding to unique collagen-rich environments within the body.
Biological function summary

CD167a/DDR1 modulates interactions between cells and their surrounding matrix. It is involved in a complex with collagen mediating signal transduction processes. This interaction is important for cellular biophysical responses driving processes like cell migration and differentiation. In marmoset thyroid tissue DDR1 helps maintain structural integrity and regulates cellular activities important for normal thyroid function.

Pathways

CD167a/DDR1 participates in key signaling pathways including those related to cell adhesion and extracellular matrix remodeling. Specifically it operates within the MAPK/ERK pathway influencing cell cycle dynamics and responses to stress signals. DDR1 also interacts with proteins like SHC and Ras enabling communication between the cell surface and intracellular targets necessary for growth and adaptation.

Mutations or irregular expression of CD167a/DDR1 have implications in cancer and fibrotic diseases. Overexpression or aberration of DDR1 can contribute to tumorigenesis facilitating tumor cell invasion and metastasis. In fibrotic conditions altered DDR1 activity can disrupt normal matrix remodeling linking it to proteins such as collagen I and III which play major roles in these disorders. Understanding DDR1's role offers potential for therapeutic interventions in targeting related pathological conditions.
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Specifications

Form

Liquid

Additional notes

The purity was determined to be >90% by densitometry. Affinity purified.

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General info

Function

Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Post-translational modifications

Autophosphorylated in response to fibrillar collagen binding.. Glycosylation of Asn-211, but apparently not of Asn-260 or Asn-394, prevents autophosphorylation from occurring in the absence of collagen.

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Product protocols

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Target data

Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
See full target information DDR1
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