Recombinant Human CD1E protein (denatured) is a Human Fragment protein, in the 30 to 305 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S E E Q L S F R M L Q T S S F A N H S W A H S E G S G W L G D L Q T H G W D T V L G T I R F L K P W S H G N F S K Q E L K N L Q S L F Q L Y F H S F I Q I V Q A S A G Q F Q L E Y P F E I Q I L A G C R M N A P Q I F L N M A Y Q G S D F L S F Q G I S W E P S P G A G I R A Q N I C K V L N R Y L D I K E I L Q S L L G H T C P R F L A G L M E A G E S E L K R K V K P E A W L S C G P S P G P G R L Q L V C H V S G F Y P K P V W V M W M R G E Q E Q R G T Q R G D V L P N A D E T W Y L R A T L D V A A G E A A G L S C R V K H S S L G G H D L I I H W G G Y S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.
Alternative names
CD1e, hCD1e, R2G1, CD1E
Recommended products
Recombinant Human CD1E protein (denatured) is a Human Fragment protein, in the 30 to 305 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P15812-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris-HCl buffer
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S E E Q L S F R M L Q T S S F A N H S W A H S E G S G W L G D L Q T H G W D T V L G T I R F L K P W S H G N F S K Q E L K N L Q S L F Q L Y F H S F I Q I V Q A S A G Q F Q L E Y P F E I Q I L A G C R M N A P Q I F L N M A Y Q G S D F L S F Q G I S W E P S P G A G I R A Q N I C K V L N R Y L D I K E I L Q S L L G H T C P R F L A G L M E A G E S E L K R K V K P E A W L S C G P S P G P G R L Q L V C H V S G F Y P K P V W V M W M R G E Q E Q R G T Q R G D V L P N A D E T W Y L R A T L D V A A G E A A G L S C R V K H S S L G G H D L I I H W G G Y S
- Accession
- P15812
- Protein length
- Fragment
- Predicted molecular weight
- 33.1 kDa
- Amino acids
- 30 to 305
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
T-cell surface glycoprotein CD1e, soluble binds diacetylated lipids, including phosphatidyl inositides and diacylated sulfoglycolipids, and is required for the presentation of glycolipid antigens on the cell surface. The membrane-associated form is not active.
- Post-translational modifications
Mono-ubiquitinated.
- Subcellular localisation
- Early endosome, Late endosome, Lysosome lumen
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD1E also known as CD1e is a member of the CD1 family of glycoproteins. It has a molecular mass of approximately 37 kDa. CD1E is expressed mainly in the endosomes of antigen-presenting cells such as dendritic cells and macrophages. It plays an essential role in lipid antigen processing inside the cells. By facilitating the presentation of lipid antigens to T cells CD1E helps modulate immune responses.
- Biological function summary
CD1E functions as part of a larger protein complex involved in the immune system. In its role it assists in the sorting and processing of lipid antigens derived from various pathogens and endogenous sources. This activity promotes the presentation of these antigens on the cell surface enabling them to be recognized by CD1-restricted T cells. Such interactions are important for developing adaptive immune responses.
- Pathways
CD1E participates in antigen processing and presentation pathways which are necessary for immune surveillance. It works closely with other CD1 family members like CD1a and CD1b to ensure the diverse display of lipid antigens to T cells. This collaboration is critical for initiating T cell-mediated immune responses against lipid-based antigens bridging innate and adaptive immunity.
- Associated diseases and disorders
CD1E has been linked to the progression and pathogenesis of certain autoimmune diseases like multiple sclerosis and rheumatoid arthritis. Dysfunction in the CD1E-mediated antigen processing pathway may lead to improper immune activation and self-reactivity. Additionally CD1E is associated with infectious diseases such as tuberculosis where it could interact with Mycobacterium tuberculosis lipids. Proteins like CD1b can influence CD1E function during these disease processes impacting the overall balance of immune responses.
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1 product image
SDS-PAGE - Recombinant Human CD1E protein (denatured) (ab177643)
15% SDS-PAGE analysis of ab177643 (3ug)
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