Recombinant Human CD20 protein is a Human Full Length protein, in the 1 to 297 aa range, expressed in Wheat germ, with >80% purity and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Purity
- >80%
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M T T P R N S V N G T F P A E P M K G P I A M Q S G P K P L F R R M S S L V G P T Q S F F M R E S K T L G A V Q I M N G L F H I A L G G L L M I P A G I Y A P I C V T V W Y P L W G G I M Y I I S G S L L A A T E K N S R K C L V K G K M I M N S L S L F A A I S G M I L S I M D I L N I K I S H F L K M E S L N F I R A H T P Y I N I Y N C E P A N P S E K N S P S T Q Y C Y S I Q S L F L G I L S V M L I F A F F Q E L V I A G I V E N E W K R T C S R P K S N I V L L S A E E K K E Q T I E I K E E V V G L T E T S S Q P K N E E D I E I I P I Q E E E E E E T E T N F P E P P Q D Q E S S P I E N D S S P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739).
Alternative names
CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1
Recommended products
Recombinant Human CD20 protein is a Human Full Length protein, in the 1 to 297 aa range, expressed in Wheat germ, with >80% purity and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
- >80%
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P11836-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M T T P R N S V N G T F P A E P M K G P I A M Q S G P K P L F R R M S S L V G P T Q S F F M R E S K T L G A V Q I M N G L F H I A L G G L L M I P A G I Y A P I C V T V W Y P L W G G I M Y I I S G S L L A A T E K N S R K C L V K G K M I M N S L S L F A A I S G M I L S I M D I L N I K I S H F L K M E S L N F I R A H T P Y I N I Y N C E P A N P S E K N S P S T Q Y C Y S I Q S L F L G I L S V M L I F A F F Q E L V I A G I V E N E W K R T C S R P K S N I V L L S A E E K K E Q T I E I K E E V V G L T E T S S Q P K N E E D I E I I P I Q E E E E E E T E T N F P E P P Q D Q E S S P I E N D S S P
- Accession
- P11836
- Protein length
- Full Length
- Predicted molecular weight
- 58.41 kDa
- Amino acids
- 1 to 297
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Glutathione Sepharose 4 Fast Flow
General info
- Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739).
- Sequence similarities
Belongs to the MS4A family.
- Post-translational modifications
Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Concentration of ab158047 is lot specific, the displayed concentration is for the available lots.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
- Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
- Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
- Associated diseases and disorders
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
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1 product image
SDS-PAGE - Recombinant Human CD20 protein (ab158047)
ab158047 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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