Recombinant human CD200R protein (Fc Chimera His Tag) is a Human Fragment protein, in the 1 to 266 aa range, expressed in HEK 293, with >94% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >94% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M L C P W R T A N L G L L L I L T I F L V A E A E G A A Q P N N S L M L Q T S K E N H A L A S S S L C M D E K Q I T Q N Y S K V L A E V N T S W P V K M A T N A V L C C P P I A L R N L I I I T W E I I L R G Q P S C T K A Y R K E T N E T K E T N C T D E R I T W V S R P D Q N S D L Q I R P V A I T H D G Y Y R C I M V T P D G N F H R G Y H L Q V L V T P E V T L F Q N R N R T A V C K A V A G K P A A Q I S W I P E G D C A T K Q E Y W S N G T V T V K S T C H W E V H N V S T V T C H V S H L T G N K S L Y I E L L P V P G A K K S A K L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
Alternative names
CD200R, CRTR2, MOX2R, OX2R, UNQ2522/PRO6015, CD200R1, Cell surface glycoprotein CD200 receptor 1, CD200 cell surface glycoprotein receptor, Cell surface glycoprotein OX2 receptor 1
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Recombinant human CD200R protein (Fc Chimera His Tag) is a Human Fragment protein, in the 1 to 266 aa range, expressed in HEK 293, with >94% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >94% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA.
Immobilized recombinant human CD200 at 1 μg/ml (100ul/well) can bind human CD200R1-Fc Chimera with a linear range of 0.12-16 ng/ml.
- Accession
- Q8TD46-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M L C P W R T A N L G L L L I L T I F L V A E A E G A A Q P N N S L M L Q T S K E N H A L A S S S L C M D E K Q I T Q N Y S K V L A E V N T S W P V K M A T N A V L C C P P I A L R N L I I I T W E I I L R G Q P S C T K A Y R K E T N E T K E T N C T D E R I T W V S R P D Q N S D L Q I R P V A I T H D G Y Y R C I M V T P D G N F H R G Y H L Q V L V T P E V T L F Q N R N R T A V C K A V A G K P A A Q I S W I P E G D C A T K Q E Y W S N G T V T V K S T C H W E V H N V S T V T C H V S H L T G N K S L Y I E L L P V P G A K K S A K L
- Accession
- Q8TD46
- Protein length
- Fragment
- Predicted molecular weight
- 54.9 kDa
- Amino acids
- 1 to 266
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
- Sequence similarities
Belongs to the CD200R family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD200R also known as CD200 receptor is a protein expressed mainly on myeloid cells including macrophages dendritic cells and certain T cells. The protein has a molecular mass of approximately 62 kDa. This receptor is a type I membrane glycoprotein that plays an important role in immune system regulation. It binds to its ligand CD200 protein found on various cell types such as neurons epithelial cells and some immune cells to mediate suppressive effects on the immune response.
- Biological function summary
CD200R plays a significant role in controlling the immune response by acting as an inhibitory receptor. When activated it sends signals that dampen immune cell activity which can prevent overactive immune responses and promote tissue homeostasis. This receptor is not part of a large receptor complex but interacts directly with its ligand CD200 to initiate signaling cascades that modulate immune functions. Its primary role lies in preventing unnecessary inflammation and autoimmunity through negative regulation of macrophage activation.
- Pathways
CD200R is involved in the regulatory pathways of the immune system. It participates in pathways that manage immune tolerance and its interactions impact pathways related to inflammation control and tissue repair. The CD200R signaling cascades intersect with pathways involving other immune-modulatory proteins such as CD200R1 and CD200R3 which contribute to cellular communication in the immune environment. These pathways play critical roles in downregulating inflammatory responses and maintaining immune balance.
- Associated diseases and disorders
CD200R holds relevance for conditions involving immune dysregulation. It is connected to autoimmune disorders and atopic dermatitis. For example in atopic dermatitis altered expression or function of CD200R may lead to improper immune responses contributing to inflammation. Furthermore CD200R agonists which are molecules that mimic the function of CD200 are an area of interest for developing therapeutic strategies to modulate immune responses. Related proteins such as CD200 and its other receptor variants play active roles in these disorders highlighting the importance of their balanced function to maintain immune homeostasis.
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2 product images
Functional Studies - Recombinant human CD200R protein (Fc Chimera His Tag) (ab276371)
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human CD200 at 1 μg/ml (100ul/well) can bind human CD200R1-Fc Chimera with a linear range of 0.12-16 ng/ml.
SDS-PAGE - Recombinant human CD200R protein (Fc Chimera His Tag) (ab276371)
SDS-PAGE analysis of ab276371.
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