Skip to main content

Recombinant human CD226 protein (Active) (Biotin) is a Human Fragment protein, in the 19 to 247 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.

Be the first to review this product! Submit a review

Images

Functional Studies - Recombinant human CD226 protein (Active) (Biotin) (AB271541), expandable thumbnail
  • SDS-PAGE - Recombinant human CD226 protein (Active) (Biotin) (AB271541), expandable thumbnail

Key facts

Purity

>=90% SDS-PAGE

Expression system

HEK 293 cells

Tags

Avi tag C-Terminus
Fc tag C-Terminus

Applications

SDS-PAGE

Biologically active

Yes

Conjugation

Biotin

Amino acid sequence

E E V L W H T S V P F A E N M S L E C V Y P S M G I L T Q V E W F K I G T Q Q D S I A I F S P T H G M V I R K P Y A E R V Y F L N S T M A S N N M T L F F R N A S E D D V G Y Y S C S L Y T Y P Q G T W Q K V I Q V V Q S D S F E A A V P S N S H I V S E P G K N V T L T C Q P Q M T W P V Q A V R W E K I Q P R Q I D L L T Y C N L V H G R N F T S K F P R Q I V S N C S H G R W S V I V I P D V T V S D S G L Y R C Y L Q A S A G E N E T F V M R L T V A E G K T D N

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed:8673704, PubMed:9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed:26755705, PubMed:31253644, PubMed:30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).

Alternative names

Recommended products

Recombinant human CD226 protein (Active) (Biotin) is a Human Fragment protein, in the 19 to 247 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.

Key facts

Purity

>=90% SDS-PAGE

Expression system

HEK 293 cells

Applications

SDS-PAGE

Biological activity

CD226, biotin binding to CD155. Briefly, 6 ng of CD226-biotin was mixed with varying amounts of CD155, His-tag in CD226 assay buffer at a final volume of 10 μL and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 mins, followed by Strep-conjugated acceptor beads for 30 mins and reading alpha-counts.

Conjugation

Biotin

Accession
Q15762-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride

Sequence info

Amino acid sequence

E E V L W H T S V P F A E N M S L E C V Y P S M G I L T Q V E W F K I G T Q Q D S I A I F S P T H G M V I R K P Y A E R V Y F L N S T M A S N N M T L F F R N A S E D D V G Y Y S C S L Y T Y P Q G T W Q K V I Q V V Q S D S F E A A V P S N S H I V S E P G K N V T L T C Q P Q M T W P V Q A V R W E K I Q P R Q I D L L T Y C N L V H G R N F T S K F P R Q I V S N C S H G R W S V I V I P D V T V S D S G L Y R C Y L Q A S A G E N E T F V M R L T V A E G K T D N

Accession
Q15762
Protein length

Fragment

Predicted molecular weight

54.7 kDa

Amino acids

19 to 247

Nature

Recombinant

Tags

Avi tag C-Terminus, Fc tag C-Terminus

Specifications

Form

Liquid

General info

Function

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed:8673704, PubMed:9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed:26755705, PubMed:31253644, PubMed:30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).

Post-translational modifications

PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment (PubMed:15684041). Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling (By similarity).

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle, Store in the dark

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Enzymatically biotin-labeled using Avi-tag™ technology

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.

Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

Associated diseases and disorders

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Recombinant human CD226 protein (Active) (Biotin) (ab271541), expandable thumbnail

    Functional Studies - Recombinant human CD226 protein (Active) (Biotin) (ab271541)

    Specific activity of ab271541.

  • SDS-PAGE - Recombinant human CD226 protein (Active) (Biotin) (ab271541), expandable thumbnail

    SDS-PAGE - Recombinant human CD226 protein (Active) (Biotin) (ab271541)

    SDS-PAGE analysis of 2 μg ab271541.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com