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Recombinant Human CD226 protein (Fc Chimera) is a Human Fragment protein, in the 19 to 247 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human CD226 protein (Fc Chimera) (AB206028), expandable thumbnail
  • Functional Studies - Recombinant Human CD226 protein (Fc Chimera) (AB206028), expandable thumbnail
  • Functional Studies - Recombinant Human CD226 protein (Fc Chimera) (AB206028), expandable thumbnail

Key facts

Purity

>95% SDS-PAGE

Endotoxin level
< 1 EU/µg
Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

E E V L W H T S V P F A E N M S L E C V Y P S M G I L T Q V E W F K I G T Q Q D S I A I F S P T H G M V I R K P Y A E R V Y F L N S T M A S N N M T L F F R N A S E D D V G Y Y S C S L Y T Y P Q G T W Q K V I Q V V Q S D S F E A A V P S N S H I V S E P G K N V T L T C Q P Q M T W P V Q A V R W E K I Q P R Q I D L L T Y C N L V H G R N F T S K F P R Q I V S N C S H G R W S V I V I P D V T V S D S G L Y R C Y L Q A S A G E N E T F V M R L T V A E G K T D N

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed:8673704, PubMed:9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed:26755705, PubMed:31253644, PubMed:30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).

Alternative names

Recommended products

Recombinant Human CD226 protein (Fc Chimera) is a Human Fragment protein, in the 19 to 247 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level
< 1 EU/µg
Expression system

HEK 293 cells

Applications

SDS-PAGE

Accession
Q15762-1
Animal free

No

Species

Human

Reconstitution

Reconstitute at 400 µg/mL in water

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: L-Arginine, Sodium chloride, 0.75% Glycine, 0.61% Tris

Sequence info

Amino acid sequence

E E V L W H T S V P F A E N M S L E C V Y P S M G I L T Q V E W F K I G T Q Q D S I A I F S P T H G M V I R K P Y A E R V Y F L N S T M A S N N M T L F F R N A S E D D V G Y Y S C S L Y T Y P Q G T W Q K V I Q V V Q S D S F E A A V P S N S H I V S E P G K N V T L T C Q P Q M T W P V Q A V R W E K I Q P R Q I D L L T Y C N L V H G R N F T S K F P R Q I V S N C S H G R W S V I V I P D V T V S D S G L Y R C Y L Q A S A G E N E T F V M R L T V A E G K T D N

Accession
Q15762
Protein length

Fragment

Predicted molecular weight

52.6 kDa

Amino acids

19 to 247

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed:8673704, PubMed:9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed:26755705, PubMed:31253644, PubMed:30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).

Post-translational modifications

PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment (PubMed:15684041). Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling (By similarity).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.

Biological function summary

CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.

Pathways

CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.

Associated diseases and disorders

CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.

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3 product images

  • SDS-PAGE - Recombinant Human CD226 protein (Fc Chimera) (ab206028), expandable thumbnail

    SDS-PAGE - Recombinant Human CD226 protein (Fc Chimera) (ab206028)

    SDS-PAGE analysis of reduced ab206028, staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 80-90 kDa in SDS-PAGE due to glycosylation.

  • Functional Studies - Recombinant Human CD226 protein (Fc Chimera) (ab206028), expandable thumbnail

    Functional Studies - Recombinant Human CD226 protein (Fc Chimera) (ab206028)

    Immobilized Rhesus macaque CD155 at 5 μg/mL (100 μL/well) can bind ab206028 with a linear range of 2-31 ng/mL.

  • Functional Studies - Recombinant Human CD226 protein (Fc Chimera) (ab206028), expandable thumbnail

    Functional Studies - Recombinant Human CD226 protein (Fc Chimera) (ab206028)

    ab206028 at 0.33μg/ml can bind 293T cells overexpressing human CD155.

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Product protocols

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