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Recombinant Human CD272/BTLA protein is a Human Fragment protein, in the 31 to 150 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
Alternative names=B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, BTLA
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, HPLC
- Biologically active
No
- Sequence
K E S C D V Q L Y I K R Q S E H S I L A G D P F E L E C P V K Y C A N R P H V T W C K L N G T T C V K L E D R Q T S W K E E K N I S F F I L H F E P V L P N D N G S Y R C S A N F Q S N L I E S H S T T L Y V T G K Q N E L S D T A G R E I N L V D H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human CD272/BTLA protein is a Human Fragment protein, in the 31 to 150 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
Alternative names=B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, BTLA
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, HPLC
- Biologically active
No
- Predicted molecular weight
14.8 kDa
- Amino acids
31 to 150
- Sequence
K E S C D V Q L Y I K R Q S E H S I L A G D P F E L E C P V K Y C A N R P H V T W C K L N G T T C V K L E D R Q T S W K E E K N I S F F I L H F E P V L P N D N G S Y R C S A N F Q S N L I E S H S T T L Y V T G K Q N E L S D T A G R E I N L V D H H H H H H
- Accession
- Q7Z6A9-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. Dissolve the lyophilized protein in 1X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. For long term storage aliquot and store at < -20°C.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride
Specifications
- Form
Lyophilized
- Additional notes
Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
General info
- Function
Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044).
- Post-translational modifications
Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
A few weeks
- Appropriate long-term storage conditions
-20°C
Notes
This product was previously labelled as CD272
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