Recombinant human CD272/BTLA protein (Fc Chimera Active) is a Human Fragment protein, in the 31 to 134 aa range, expressed in HEK 293, with >92% purity, < 1 EU/µg endotoxin level and suitable for FuncS, SDS-PAGE.
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Key facts
- Purity
- >92% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
K E S C D V Q L Y I K R Q S E H S I L A G D P F E L E C P V K Y C A N R P H V T W C K L N G T T C V K L E D R Q T S W K E E K N I S F F I L H F E P V L P N D N G S Y R C S A N F Q S N L I E S H S T T L Y V T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes The reducing protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation. |
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Target data
Function
Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044).
Alternative names
CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, BTLA
Recommended products
Recombinant human CD272/BTLA protein (Fc Chimera Active) is a Human Fragment protein, in the 31 to 134 aa range, expressed in HEK 293, with >92% purity, < 1 EU/µg endotoxin level and suitable for FuncS, SDS-PAGE.
Key facts
- Purity
- >92% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- FuncS, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind ab219719 with a linear range of 0.02-1.25 μg/mL.
- Accession
- Q7Z6A9-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 0.75% Glycine, 0.61% Tris buffer
Sequence info
Amino acid sequence
- K E S C D V Q L Y I K R Q S E H S I L A G D P F E L E C P V K Y C A N R P H V T W C K L N G T T C V K L E D R Q T S W K E E K N I S F F I L H F E P V L P N D N G S Y R C S A N F Q S N L I E S H S T T L Y V T
- Accession
- Q7Z6A9
- Protein length
- Fragment
- Predicted molecular weight
- 38.7 kDa
- Amino acids
- 31 to 134
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044).
- Post-translational modifications
Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as CD272
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD272 also known as BTLA (B and T Lymphocyte Attenuator) is an immunoregulatory protein with a mass approximately 34 kDa. This target is part of the CD28 immunoglobulin superfamily and is expressed on the surfaces of various immune cells including T cells B cells and dendritic cells. BTLA/CD272 serves as a co-inhibitory molecule important in maintaining immune homeostasis. Its expression is influenced by factors present in the microenvironment of immune tissues.
- Biological function summary
BTLA functions as a negative regulator of immune responses by delivering inhibitory signals to immune cells upon binding to its ligand HVEM (Herpesvirus entry mediator). It forms a part of an immunological checkpoint complex that modulates lymphocyte activity to prevent overactivation and autoimmunity. This mechanism helps maintain a balance between immune defense and tolerance contributing to self-tolerance.
- Pathways
The BTLA/CD272 protein engages with pathways involved in immune checkpoints and T-cell receptor signaling. It interacts with proteins like HVEM and collaborates with receptors such as PD-1 and CTLA-4. These interactions play a role in the downregulation of T-cell responses which is essential in various immune processes. The signaling pathways influenced by BTLA/CD272 are integral in moderating immune responses cooperation and adaptation.
- Associated diseases and disorders
BTLA is associated with autoimmune diseases and cancer. In autoimmune disorders an altered expression or function of BTLA can lead to dysregulated immune responses causing tissue damage. In cancer the protein may contribute to immune evasion by tumors aiding tumor growth and progression. The interplay of BTLA with proteins such as PD-1 makes it a potential target for immunotherapy strategies in oncology and autoimmunity treatments.
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2 product images
Functional Studies - Recombinant human CD272/BTLA protein (Fc Chimera Active) (ab219719)
Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind ab219719 with a linear range of 0.02-1.25 μg/mL.
SDS-PAGE - Recombinant human CD272/BTLA protein (Fc Chimera Active) (ab219719)
SDS-PAGE analysis of reduced ab219719 stained overnight with Coomassie Blue.
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