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AB307810

Recombinant Human B7-H3/CD276 Protein

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Recombinant Human B7-H3/CD276 Protein is a Human Fragment protein, in the 29 to 245 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, SDS-PAGE, Mass Spec.
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Mass Spectrometry - Recombinant Human B7-H3/CD276 Protein (AB307810)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human B7-H3/CD276 Protein (AB307810)

Mass determination by ESI-TOF. Predicted MW is 23351.36 Da (+/- 10 Da by ESI-TOF). Observed MW is 23368.18 Da.

SDS-PAGE - Recombinant Human B7-H3/CD276 Protein (AB307810)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human B7-H3/CD276 Protein (AB307810)

SDS-PAGE analysis of ab307810

HPLC - Recombinant Human B7-H3/CD276 Protein (AB307810)
  • HPLC

Supplier Data

HPLC - Recombinant Human B7-H3/CD276 Protein (AB307810)

HPLC analysis of ab307810

Key facts

Purity

>95% HPLC, SDS-PAGE

SDS-PAGE >= 95%

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, HPLC, SDS-PAGE

applications

Biologically active

No

Accession

Q5ZPR3-2

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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Proteins & Peptides

AB280753

Recombinant Human PD-1/CD279 protein

Mass Spectrometry - Recombinant Human PD-1/CD279 protein (AB280753)

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Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

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Primary Antibodies

AB134161

Anti-B7-H3/CD276 antibody [EPNCIR122]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-B7-H3/CD276 antibody [EPNCIR122] (AB134161)

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  • 0
Proteins & Peptides

AB276831

Recombinant Mouse TIM 4 protein (His tag)

SDS-PAGE - Recombinant Mouse TIM 4 protein (His tag) (AB276831)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"LEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSILRVVLGANGTYSCLVRNPVLQQDAHSSVTITPQRSPTG","proteinLength":"Fragment","predictedMolecularWeight":"23.35 kDa","actualMolecularWeight":"23.37 kDa","aminoAcidEnd":245,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q5ZPR3","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD276 also known as B7-H3 is an immunoregulatory protein with a mass of approximately 57 kDa although post-translational modifications can increase its observed size. It is a member of the B7 family which is part of the immunoglobulin superfamily. CD276 is widely expressed in various tissues including placenta liver heart and also in many tumor cells. It is observed in lesser amounts in normal tissues making it an interesting target for cancer research. Various assays like HEK293 and HEK293T cell confluency studies often use CD276 to explore its expression under different conditions and seeding densities which are represented in confluency charts.
Biological function summary

CD276 modulates immune responses by acting as a co-inhibitory ligand impacting T-cell proliferation and cytokine synthesis. Not part of a known complex it plays a role in the adaptive immune system by providing negative feedback regulation that can inhibit the immune response. This capability has implications in preventing autoimmunity but may also contribute to tumor immune evasion by reducing effective anti-tumor responses. The B7-H3/CD276 interaction with receptors such as IL receptor family members suggests its diverse immunomodulatory functions.

Pathways

CD276 participates in the T-cell receptor (TCR) signaling pathways influencing immune checkpoint pathways. It interacts with other immune checkpoint proteins like PD-L1 and CTLA-4 but its exact receptor partner remains unidentified. The presence of CD276 in these pathways highlights its role in controlling T-cell activation and tolerance. By modulating TCR signals and impacting downstream effects CD276 serves as a regulatory point that can balance immune activation and inhibition critical in immune-mediated conditions.

CD276 has strong associations with cancer particularly in solid tumors such as prostate and breast cancer. It is often overexpressed in malignant tissues contributing to immune evasion by tumors. Researchers propose it as a potential target for cancer immunotherapy due to this attribute. CD276's link to disorders like asthma has also been suggested where its interaction with proteins involved in inflammatory pathways such as IL response elements can exacerbate the condition. This implicates it as a dual-role protein in both disease progression and potential therapeutic targeting.
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Product protocols

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Target data

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
See full target information B7-H3/CD276

Alternative Names

CD276, B7H3, PSEC0249, UNQ309/PRO352, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, B7-H3
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Product promise

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