Recombinant human CD28 protein (Active)
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Recombinant human CD28 protein (Active) is a Human Fragment protein, in the 19 to 152 aa range, expressed in HEK 293 cells, with >95%, < 0.2 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
View Alternative Names
CD28, T-cell-specific surface glycoprotein CD28, TP44
- ELISA
Supplier Data
ELISA - Recombinant human CD28 protein (Active) (AB286013)
CD28 and CD80-Biotin ELISA binding activity :
Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 μg/mL, 100 ng/well with a linear range of 0.03 – 0.81 μg/mL.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human CD28 protein (Active) (AB286013)
SDS-PAGE (4-20%) : Human CD28, Fc-Tag protein was run on SDS PAGE gel under reducing (R) conditions and stained with Coomassie Blue.
Lane M : Protein Marker
Lane 1 : Biotinylated Human CD28 Fc Tag (2 μg)
Lane 2 : Human CD28 Fc Tag (2 μg).
The proteins migrate to around 55 kDa due to glycosylation.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.
Pathways
CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.
Specifications
Form
Lyophilized
General info
Function
Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).
Post-translational modifications
Phosphorylated by LCK (PubMed:7568038). Dephosphorylated by PTPN11 (PubMed:36114179).. Isoform 3. Tyrosine phosphorylated induced by CD40LG.
Target data
Complementary Products
Product promise
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