Recombinant human CD28 protein (Active) is a Human Fragment protein, in the 19 to 152 aa range, expressed in HEK 293, with >=95% purity, < 0.2 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
< 0.2 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Alternative names
T-cell-specific surface glycoprotein CD28, TP44, CD28
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Recombinant human CD28 protein (Active) is a Human Fragment protein, in the 19 to 152 aa range, expressed in HEK 293, with >=95% purity, < 0.2 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Alternative names
T-cell-specific surface glycoprotein CD28, TP44, CD28
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
< 0.2 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 μg/mL, 100 μL/well with a linear range of 0.03 – 0.81 μg/mL.
- Accession
- P10747-1
- Animal free
No
- Species
Human
- Reconstitution
Reconstitute in sterile PBS, pH 7.4 to a concentration of 10 µg/ml
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- Accession
- Protein length
Fragment
- Predicted molecular weight
25 kDa
- Amino acids
19 to 152
- Nature
Recombinant
- Tags
Fc tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
- Post-translational modifications
CD40LG induces tyrosine phosphorylation of isoform 3.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 9237 Human CellExp™ CD28, Human Recombinant. 9237-100 is the same size as the 100 μg size of ab286013.
Supplementary info
- Activity summary
CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
- Biological function summary
CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.
- Pathways
CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.
- Associated diseases and disorders
CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.
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2 product images
ELISA - Recombinant human CD28 protein (Active) (ab286013)
CD28 and CD80-Biotin ELISA binding activity:
Human biotinylated CD80, Fc Tag can bind to immobilized human CD28, Fc Tag at 1 μg/mL, 100 ng/well with a linear range of 0.03 – 0.81 μg/mL.
SDS-PAGE - Recombinant human CD28 protein (Active) (ab286013)
SDS-PAGE (4-20%): Human CD28, Fc-Tag protein was run on SDS PAGE gel under reducing (R) conditions and stained with Coomassie Blue.
Lane M: Protein Marker
Lane 1: Biotinylated Human CD28 Fc Tag (2 μg)
Lane 2: Human CD28 Fc Tag (2 μg).
The proteins migrate to around 55 kDa due to glycosylation.
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