Recombinant human CD3 epsilon + CD3D protein (Active)
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Recombinant human CD3 epsilon + CD3D protein (Active) is a Human Fragment protein, in the 23 to 126 aa range, expressed in Expi293 F cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, ELISA.
View Alternative Names
CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
- ELISA
Supplier Data
ELISA - Recombinant human CD3 epsilon + CD3D protein (Active) (AB286041)
ab286041 was immobilized at 0.5 μg/ml (100 μl/well). Dose response curve using Anti-hCD3E/CD3D ab1 foralumab gave an EC50 of 21.69 ng/ml by ELISA
- ELISA
Supplier Data
ELISA - Recombinant human CD3 epsilon + CD3D protein (Active) (AB286041)
ab286041 was Immobilized at 0.1 μg/ml (100 μl/Well). Dose response curve using Anti-hCD3E/CD3D ab2 OKT3 gave an EC50 of 21 ng/ml by ELISA.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human CD3 epsilon + CD3D protein (Active) (AB286041)
ab286041 run on Tris-Bis PAGE gel under reducing conditions. The protein migrates as 40-50 kDa due to glycosylation.
Complementary Products
Reagents, Controls & Accessories
AB313996
Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)
- 0
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Reactivity data
Product details
This product is manufactured by BioVision, an Abcam company and was previously called P1183 Human CellExp™ CD3 epsilon & CD3 delta Heterodimer. P1183-10 is the same size as the 10 μg size of ab286041.
Reconstituted protein stable at -80°C for 3 months. Avoid freeze / thaw cycle.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Specifications
Form
Lyophilized
General info
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
Post-translational modifications
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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