Recombinant human CD3 epsilon + CD3D protein (Active) is a Human Fragment protein, in the 23 to 126 aa range, expressed in Expi293 F, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Expi293 F cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS, ELISA
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).
Additional Targets
CD3D, CD3D
Alternative names
CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
Recommended products
Recombinant human CD3 epsilon + CD3D protein (Active) is a Human Fragment protein, in the 23 to 126 aa range, expressed in Expi293 F, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Expi293 F cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS, ELISA
- Biologically active
- Yes
- Biological activity
- Human CD3 epsilon and CD3 delta Heterodimer Protein was immobilized at 0.5 μg/ml (100 μl/well). Dose response curve using Anti-hCD3E/CD3D Ab foralumab gave an EC50 of 21.69 ng/ml by ELISA. Human CD3 epsilon and CD3 delta Heterodimer Protein was Immobilized at 0.1 μg/ml (100 μl/Well). Dose response curve using Anti-hCD3E/CD3D Ab OKT3 gave an EC50 of 21 ng/ml by ELISA.
- Accession
- P04234-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 99% PBS
Sequence info
Amino acid sequence 1
- Accession
- P07766
- Protein length
- Fragment
- Predicted molecular weight
- 37.8 kDa
- Amino acids
- 23 to 126
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Amino acid sequence 2
- Accession
- P04234
- Protein length
- Fragment
- Predicted molecular weight
- 35.4 kDa
- Amino acids
- 22 to 105
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).
- Post-translational modifications
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is manufactured by BioVision, an Abcam company and was previously called P1183 Human CellExp™ CD3 epsilon & CD3 delta Heterodimer. P1183-10 is the same size as the 10 μg size of ab286041.
Reconstituted protein stable at -80°C for 3 months. Avoid freeze / thaw cycle.
Product promise
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3 product images
ELISA - Recombinant human CD3 epsilon + CD3D protein (Active) (ab286041)
ab286041 was Immobilized at 0.1 μg/ml (100 μl/Well). Dose response curve using Anti-hCD3E/CD3D ab2 OKT3 gave an EC50 of 21 ng/ml by ELISA.
ELISA - Recombinant human CD3 epsilon + CD3D protein (Active) (ab286041)
ab286041 was immobilized at 0.5 μg/ml (100 μl/well). Dose response curve using Anti-hCD3E/CD3D Anti-HIF-1 alpha antibody [H1alpha67] ab1 foralumab gave an EC50 of 21.69 ng/ml by ELISA
SDS-PAGE - Recombinant human CD3 epsilon + CD3D protein (Active) (ab286041)
ab286041 run on Tris-Bis PAGE gel under reducing conditions. The protein migrates as 40-50 kDa due to glycosylation.
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