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Recombinant human CD3 epsilon protein (Active) is a Human Fragment protein, in the 23 to 126 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE and Functional studies.The predicted molecular weight of ab167755 protein is 16.9 kDa.

- Save time and ensure accurate results - use our recombinant human CD3 epsilon /CD3e protein as a control
- Optimal protein bioactivity and stability
- ab167755 protein is designed as a dimer

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Images

SDS-PAGE - Recombinant human CD3 epsilon protein (Active) (AB167755), expandable thumbnail
  • Size Exclusion Chromatography - Recombinant human CD3 epsilon protein (Active) (AB167755), expandable thumbnail
  • Functional Studies - Recombinant human CD3 epsilon protein (Active) (AB167755), expandable thumbnail

Key facts

Purity
>90% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Tags
His tag C-Terminus
Applications
SDS-PAGE, FuncS
Biologically active
Yes

Amino acid sequence

D G N E E M G G I T Q T P Y K V S I S G T T V I L T C P Q Y P G S E I L W Q H N D K N I G G D E D D K N I G S D E D H L S L K E F S E L E Q S G Y Y V C Y P R G S K P E D A N F Y L Y L R A R V C E N C M E M D

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

As a result of glycosylation, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).

Alternative names

Recommended products

Recombinant human CD3 epsilon protein (Active) is a Human Fragment protein, in the 23 to 126 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE and Functional studies.The predicted molecular weight of ab167755 protein is 16.9 kDa.

- Save time and ensure accurate results - use our recombinant human CD3 epsilon /CD3e protein as a control
- Optimal protein bioactivity and stability
- ab167755 protein is designed as a dimer

Key facts

Purity
>90% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE, FuncS
Biological activity
Immobilized Mouse Anti-Human CD3 at 2 μg/mL (100 μL/well) binds ab167755.

Linear range: 0.2-2 ng/mL (QC tested).

Accession
P07766-1
Animal free
No
Carrier free
Yes
Species
Human
Reconstitution
Reconstitute with sterile deionized water to a concentration of 600 µg/ml.
Concentration
Loading...
Storage buffer

pH: 7.54
Constituents: 100% PBS

Sequence info

Amino acid sequence

D G N E E M G G I T Q T P Y K V S I S G T T V I L T C P Q Y P G S E I L W Q H N D K N I G G D E D D K N I G S D E D H L S L K E F S E L E Q S G Y Y V C Y P R G S K P E D A N F Y L Y L R A R V C E N C M E M D
Accession
P07766
Protein length
Fragment
Predicted molecular weight
16.9 kDa
Amino acids
23 to 126
Nature
Recombinant
Tags
His tag C-Terminus

Specifications

Form
Lyophilized
Additional notes

Purified by Immobilized metal affinity chromatography.

General info

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).

Post-translational modifications

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA), Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Ensure the validity of your result using our bioactive recombinant human CD3 epsilon /CD3e protein ab167755 as control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • SDS-PAGE - Recombinant human CD3 epsilon protein (Active) (ab167755), expandable thumbnail

    SDS-PAGE - Recombinant human CD3 epsilon protein (Active) (ab167755)

    ab167755 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Size Exclusion Chromatography - Recombinant human CD3 epsilon protein (Active) (ab167755), expandable thumbnail

    Size Exclusion Chromatography - Recombinant human CD3 epsilon protein (Active) (ab167755)

    The purity of Human CD3 epsilon, His Tag (ab167755) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

  • Functional Studies - Recombinant human CD3 epsilon protein (Active) (ab167755), expandable thumbnail

    Functional Studies - Recombinant human CD3 epsilon protein (Active) (ab167755)

    Immobilized Mouse Anti Human CD3 at 2 μg/mL (100 μL/well) can bind ab167755 with a linear range of 0.2-2 ng/mL (QC tested).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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