Recombinant human CD3 epsilon protein (Active)
- Bioactive
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Recombinant human CD3 epsilon protein (Active) is a Human Fragment protein, in the 23 to 126 aa range, expressed in HEK 293, with >90% purity, <1 EU/µg endotoxin level and suitable for SDS-PAGE and Functional studies.The predicted molecular weight of ab167755 protein is 16.9 kDa.
- Save time and ensure accurate results - use our recombinant human CD3 epsilon /CD3e protein as a control
- Optimal protein bioactivity and stability
- ab167755 protein is designed as a dimer
View Alternative Names
CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain
- FuncS
Supplier Data
Functional Studies - Recombinant human CD3 epsilon protein (Active) (AB167755)
Immobilized Mouse Anti Human CD3 at 2 μg/mL (100 μL/well) can bind ab167755 with a linear range of 0.2-2 ng/mL (QC tested).
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human CD3 epsilon protein (Active) (AB167755)
ab167755 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- Size Exclusion Chromatography
Supplier Data
Size Exclusion Chromatography - Recombinant human CD3 epsilon protein (Active) (AB167755)
The purity of Human CD3 epsilon, His Tag (ab167755) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
Complementary Products
Reagents, Controls & Accessories
AB234592
Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)
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Reactivity data
Product details
Check out our protein gel staining guide for SDS-PAGE here
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Specifications
Form
Lyophilized
Additional notes
Purified by Immobilized metal affinity chromatography.
General info
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response (PubMed : 15294938, PubMed : 15546002, PubMed : 2470098, PubMed : 40592325, PubMed : 8490660). When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z (PubMed : 2470098, PubMed : 40592325). All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain (PubMed : 2470098, PubMed : 40592325). Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098, PubMed : 40592325). CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (By similarity). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (By similarity). Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1 (PubMed : 38614099). Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 12110186, PubMed : 38614099).
Post-translational modifications
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Target data
Product promise
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