Recombinant Human CD3 epsilon protein (Fc Chimera His Tag) is a Human Fragment protein, in the 23 to 126 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
D G N E E M G G I T Q T P Y K V S I S G T T V I L T C P Q Y P G S E I L W Q H N D K N I G G D E D D K N I G S D E D H L S L K E F S E L E Q S G Y Y V C Y P R G S K P E D A N F Y L Y L R A R V C E N C M E M D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).
Alternative names
CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain
Recommended products
Recombinant Human CD3 epsilon protein (Fc Chimera His Tag) is a Human Fragment protein, in the 23 to 126 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 μg/mL (100 μL/well) can bind ab220590 with a linear range of 0.1-3 ng/mL (Routinely tested).
- Accession
- P07766-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.54
Constituents: 100% PBS
Sequence info
Amino acid sequence
- D G N E E M G G I T Q T P Y K V S I S G T T V I L T C P Q Y P G S E I L W Q H N D K N I G G D E D D K N I G S D E D H L S L K E F S E L E Q S G Y Y V C Y P R G S K P E D A N F Y L Y L R A R V C E N C M E M D
- Accession
- P07766
- Protein length
- Fragment
- Predicted molecular weight
- 39.2 kDa
- Amino acids
- 23 to 126
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).
- Post-translational modifications
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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2 product images
SDS-PAGE - Recombinant Human CD3 epsilon protein (Fc Chimera His Tag) (ab220590)
SDS-PAGE analysis of reduced ab220590 stained overnight with Coomassie Blue.
The protein migrates as 44-54 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
Functional Studies - Recombinant Human CD3 epsilon protein (Fc Chimera His Tag) (ab220590)
Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 μg/mL (100 μL/well) can bind ab220590 with a linear range of 0.1-3 ng/mL (Routinely tested).
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