Recombinant Human CD33 protein (Tagged) is a Human Fragment protein, in the 1 to 259 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M P L L L L L P L L W A G A L A M D P N F W L Q V Q E S V T V Q E G L C V L V P C T F F H P I P Y Y D K N S P V H G Y W F R E G A I I S R D S P V A T N K L D Q E V Q E E T Q G R F R L L G D P S R N N C S L S I V D A R R R D N G S Y F F R M E R G S T K Y S Y K S P Q L S V H V T D L T H R P K I L I P G T L E P G H S K N L T C S V S W A C E Q G T P P I F S W L S A A P T S L G P R T T H S S V L I I T P R P Q D H G T N L T C Q V K F A G A G V T T E R T I Q L N V T Y V P Q N P T T G I F P G D G S G K Q E T R A G V V H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:11320212, PubMed:15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:10887109, PubMed:28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10206955, PubMed:10556798, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).
Alternative names
CD33, SIGLEC3, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, gp67, Siglec-3
Recommended products
Recombinant Human CD33 protein (Tagged) is a Human Fragment protein, in the 1 to 259 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P20138-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M P L L L L L P L L W A G A L A M D P N F W L Q V Q E S V T V Q E G L C V L V P C T F F H P I P Y Y D K N S P V H G Y W F R E G A I I S R D S P V A T N K L D Q E V Q E E T Q G R F R L L G D P S R N N C S L S I V D A R R R D N G S Y F F R M E R G S T K Y S Y K S P Q L S V H V T D L T H R P K I L I P G T L E P G H S K N L T C S V S W A C E Q G T P P I F S W L S A A P T S L G P R T T H S S V L I I T P R P Q D H G T N L T C Q V K F A G A G V T T E R T I Q L N V T Y V P Q N P T T G I F P G D G S G K Q E T R A G V V H
- Accession
- P20138
- Protein length
- Fragment
- Predicted molecular weight
- 57 kDa
- Amino acids
- 1 to 259
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, Fc tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:11320212, PubMed:15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:10887109, PubMed:28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10206955, PubMed:10556798, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).
- Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
- Post-translational modifications
Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition.
- Subcellular localisation
- Peroxisome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human CD33 protein (Tagged) (ab271427)
SDS-PAGE analysis of ab271427.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com