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AB269982

Recombinant human CD38 protein (Active) (APC)

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Recombinant human CD38 protein (Active) (APC) is a Human Fragment protein, in the 43 to 300 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1

2 Images
Functional Studies - Recombinant human CD38 protein (Active) (APC) (AB269982)
  • FuncS

Supplier Data

Functional Studies - Recombinant human CD38 protein (Active) (APC) (AB269982)

Functional analysis of ab269982.

Add CD38 enzyme to 50 μl reaction mix containing NGD+ (Nicotinamide Guanine Dinucleotide). Incubate 4 min at room temperature. Read fluorescence (λ exc=300 nm, λ em=410 nm).

SDS-PAGE - Recombinant human CD38 protein (Active) (APC) (AB269982)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human CD38 protein (Active) (APC) (AB269982)

SDS-PAGE analysis of ab269982 ona 4-20% gel with Coomassie staining.

Lane 1 : ab269982 (8 μg).

Lane 2 : Unlabeled CD38 (4 μg).

Lane 3 : APC (4 μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Assay conditions: Add CD38 enzyme to 50 μl reaction mix containing NGD+ (Nicotinamide Guanine Dinucleotide). Incubate 4 min at room temperature. Read fluorescence (λ exc=300 nm, λ em=410 nm).

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Accession

P28907

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.
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Sequence info

[{"sequence":"VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI","proteinLength":"Fragment","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":300,"aminoAcidStart":43,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P28907","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle|Store in the dark
True
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Specifications

Form

Liquid

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General info

Function

Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed : 7961800, PubMed : 8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed : 16690024). Has cADPR hydrolase activity (PubMed : 7961800, PubMed : 8253715).

Sequence similarities

Belongs to the ADP-ribosyl cyclase family.

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Product protocols

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Target data

Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed : 7961800, PubMed : 8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed : 16690024). Has cADPR hydrolase activity (PubMed : 7961800, PubMed : 8253715).
See full target information CD38
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