Recombinant human CD39 protein (Active) is a Human Fragment protein, in the 38 to 478 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E D T K E S N V K T F C S K N I L A I L G F S S I I A V I A L L A V G L T Q N K A L P E N V K Y G I V L D A G S S H T S L Y I Y K W P A E K E N D T G V V H Q V E E C R V K G P G I S K F V Q K V N E I G I Y L T D C M E R A R E V I P R S Q H Q E T P V Y L G A T A G M R L L R M E S E E L A D R V L D V V E R S L S N Y P F D F Q G A R I I T G Q E E G A Y G W I T I N Y L L G K F S Q K T R W F S I V P Y E T N N Q E T F G A L D L G G A S T Q V T F V P Q N Q T I E S P D N A L Q F R L Y G K D Y N V Y T H S F L C Y G K D Q A L W Q K L A K D I Q V A S N E I L R D P C F H P G Y K K V V N V S D L Y K T P C T K R F E M T L P F Q Q F E I Q G I G N Y Q Q C H Q S I L E L F N T S Y C P Y S Q C A F N G I F L P P L Q G D F G A F S A F Y F V M K F L N L T S E K V S Q E K V T E M M K K F C A Q P W E E I K T S Y A G V K E K Y L S E Y C F S G T Y I L S L L L Q G Y H F T A D S W E H I H F I G K I Q G S D A G W T L G Y M L N L T N M I P A E Q P L S T P L S H S T Y V F L M V L F S L V L F T V A I I G L L I F H K P S Y F W K D M V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the hydrolysis of both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates and participates in the regulation of extracellular levels of nucleotides (Probable) (PubMed:8529670, PubMed:8626624, PubMed:8955160, PubMed:8996251). By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow (PubMed:8955160, PubMed:8996251).
Alternative names
CD39, ENTPD1, Ectonucleoside triphosphate diphosphohydrolase 1, ATP diphosphohydrolase, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen, Nucleoside triphosphate diphosphohydrolase 1, ATP-DPH, ATPDase, Ecto-ATPDase 1, Ecto-ATPase 1, NTPDase1
Recommended products
Recombinant human CD39 protein (Active) is a Human Fragment protein, in the 38 to 478 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity >3000 pmol/min/μg.
Colorimetric ATP hydrolysis assay.
- Accession
- P49961-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M E D T K E S N V K T F C S K N I L A I L G F S S I I A V I A L L A V G L T Q N K A L P E N V K Y G I V L D A G S S H T S L Y I Y K W P A E K E N D T G V V H Q V E E C R V K G P G I S K F V Q K V N E I G I Y L T D C M E R A R E V I P R S Q H Q E T P V Y L G A T A G M R L L R M E S E E L A D R V L D V V E R S L S N Y P F D F Q G A R I I T G Q E E G A Y G W I T I N Y L L G K F S Q K T R W F S I V P Y E T N N Q E T F G A L D L G G A S T Q V T F V P Q N Q T I E S P D N A L Q F R L Y G K D Y N V Y T H S F L C Y G K D Q A L W Q K L A K D I Q V A S N E I L R D P C F H P G Y K K V V N V S D L Y K T P C T K R F E M T L P F Q Q F E I Q G I G N Y Q Q C H Q S I L E L F N T S Y C P Y S Q C A F N G I F L P P L Q G D F G A F S A F Y F V M K F L N L T S E K V S Q E K V T E M M K K F C A Q P W E E I K T S Y A G V K E K Y L S E Y C F S G T Y I L S L L L Q G Y H F T A D S W E H I H F I G K I Q G S D A G W T L G Y M L N L T N M I P A E Q P L S T P L S H S T Y V F L M V L F S L V L F T V A I I G L L I F H K P S Y F W K D M V
- Accession
- P49961
- Protein length
- Fragment
- Predicted molecular weight
- 51 kDa
- Amino acids
- 38 to 478
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the hydrolysis of both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates and participates in the regulation of extracellular levels of nucleotides (Probable) (PubMed:8529670, PubMed:8626624, PubMed:8955160, PubMed:8996251). By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow (PubMed:8955160, PubMed:8996251).
- Sequence similarities
Belongs to the GDA1/CD39 NTPase family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Recombinant human CD39 protein (Active) (ab271430)
Functional analysis of ab271430.
Specific activity >3000 pmol/min/μg.
Colorimetric ATP hydrolysis assay.
SDS-PAGE - Recombinant human CD39 protein (Active) (ab271430)
SDS-PAGE analysis of ab271430.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com