Recombinant Human CD40 Ligand Protein (His-tag)
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human CD40 Ligand Protein (His-tag) is a Human Fragment protein, expressed in HEK 293 cells, with >95%, <2.16 EU/mg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE.
View Alternative Names
CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Human CD40 Ligand Protein (His-tag) (AB317872)
Mass determination by ESI-TOF. Predicted MW is 25870 Da (+/- 10 Da by ESI-TOF). Observed MW is 25874.03 Da.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human CD40 Ligand Protein (His-tag) (AB317872)
SDS-PAGE analysis of ab317872 under reducing conditions for 2ug protein.
- HPLC
Supplier Data
HPLC - Recombinant Human CD40 Ligand Protein (His-tag) (AB317872)
HPLC analysis of ab317872
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.
Pathways
CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.
Specifications
Form
Lyophilized
General info
Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed : 1280226, PubMed : 31331973). Costimulates T-cell proliferation and cytokine production (PubMed : 8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed : 8617933). Induces the activation of NF-kappa-B (PubMed : 15067037, PubMed : 31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed : 15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).. CD40 ligand, soluble form. Acts as a ligand for integrins, specifically ITGA5 : ITGB1 and ITGAV : ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Sequence similarities
Belongs to the tumor necrosis factor family.
Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing.. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.. Not O-glycosylated.
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com