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Recombinant human CD40 protein (Active) is a Human Fragment protein, in the 21 to 193 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

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Images

SDS-PAGE - Recombinant human CD40 protein (Active) (AB155710), expandable thumbnail
  • ELISA - Recombinant human CD40 protein (Active) (AB155710), expandable thumbnail
  • Functional Studies - Recombinant human CD40 protein (Active) (AB155710), expandable thumbnail

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Tags
Fc tag C-Terminus
Applications
SDS-PAGE, ELISA, FuncS
Biologically active
Yes

Amino acid sequence

E P P T A C R E K Q Y L I N S Q C C S L C Q P G Q K L V S D C T E F T E T E C L P C G E S E F L D T W N R E T H C H Q H K Y C D P N L G L R V Q Q K G T S E T D T I C T C E E G W H C T S E A C E S C V L H R S C S P G F G V K Q I A T G V S D T I C E P C P V G F F S N V S S A F E K C H P W T S C E T K D L V V Q Q A G T N K T D V V C G P Q D R L R

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).

Alternative names

Recommended products

Recombinant human CD40 protein (Active) is a Human Fragment protein, in the 21 to 193 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE, ELISA, FuncS
Biological activity
Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind ab155710 with a linear range of 0.039-1.25 μg/mL.
Accession
P25942-1
Animal free
No
Species
Human
Reconstitution
Reconstitute at 400 µg/mL in water
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris

Sequence info

Amino acid sequence

E P P T A C R E K Q Y L I N S Q C C S L C Q P G Q K L V S D C T E F T E T E C L P C G E S E F L D T W N R E T H C H Q H K Y C D P N L G L R V Q Q K G T S E T D T I C T C E E G W H C T S E A C E S C V L H R S C S P G F G V K Q I A T G V S D T I C E P C P V G F F S N V S S A F E K C H P W T S C E T K D L V V Q Q A G T N K T D V V C G P Q D R L R
Accession
P25942
Protein length
Fragment
Predicted molecular weight
45.3 kDa
Amino acids
21 to 193
Nature
Recombinant
Tags
Fc tag C-Terminus

Specifications

Form
Lyophilized
Additional notes

>90% as determined by SEC-HPLC.Protein A purified.

General info

Function

Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Measured by its binding ability in a functional ELISA. Immobilized ab155710 at 2 µg/ml (100 µl/well) can bind biotinylated Human CD40L with a linear range of 7.8 - 125 ng/ml.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • SDS-PAGE - Recombinant human CD40 protein (Active) (ab155710), expandable thumbnail

    SDS-PAGE - Recombinant human CD40 protein (Active) (ab155710)

    SDS-PAGE of reduced ab155710 stained overnight with Coomassie Blue.

  • ELISA - Recombinant human CD40 protein (Active) (ab155710), expandable thumbnail

    ELISA - Recombinant human CD40 protein (Active) (ab155710)

    Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind ab155710 with a linear range of 0.039-1.25 μg/mL.

  • Functional Studies - Recombinant human CD40 protein (Active) (ab155710), expandable thumbnail

    Functional Studies - Recombinant human CD40 protein (Active) (ab155710)

    ab155710 at 0.1 μg/ml can bind to 293T cells overexpressing human CD40L as determined by FACS analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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