Recombinant human CD40 protein (Active) is a Human Fragment protein, in the 21 to 193 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, FuncS
- Biologically active
- Yes
Amino acid sequence
E P P T A C R E K Q Y L I N S Q C C S L C Q P G Q K L V S D C T E F T E T E C L P C G E S E F L D T W N R E T H C H Q H K Y C D P N L G L R V Q Q K G T S E T D T I C T C E E G W H C T S E A C E S C V L H R S C S P G F G V K Q I A T G V S D T I C E P C P V G F F S N V S S A F E K C H P W T S C E T K D L V V Q Q A G T N K T D V V C G P Q D R L R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Alternative names
CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Recommended products
Recombinant human CD40 protein (Active) is a Human Fragment protein, in the 21 to 193 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, FuncS
- Biologically active
- Yes
- Biological activity
- Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind ab155710 with a linear range of 0.039-1.25 μg/mL.
- Accession
- P25942-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris
Sequence info
Amino acid sequence
- E P P T A C R E K Q Y L I N S Q C C S L C Q P G Q K L V S D C T E F T E T E C L P C G E S E F L D T W N R E T H C H Q H K Y C D P N L G L R V Q Q K G T S E T D T I C T C E E G W H C T S E A C E S C V L H R S C S P G F G V K Q I A T G V S D T I C E P C P V G F F S N V S S A F E K C H P W T S C E T K D L V V Q Q A G T N K T D V V C G P Q D R L R
- Accession
- P25942
- Protein length
- Fragment
- Predicted molecular weight
- 45.3 kDa
- Amino acids
- 21 to 193
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant human CD40 protein (Active) (ab155710)
SDS-PAGE of reduced ab155710 stained overnight with Coomassie Blue.
ELISA - Recombinant human CD40 protein (Active) (ab155710)
Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind ab155710 with a linear range of 0.039-1.25 μg/mL.
Functional Studies - Recombinant human CD40 protein (Active) (ab155710)
ab155710 at 0.1 μg/ml can bind to 293T cells overexpressing human CD40L as determined by FACS analysis.
Downloads
Product protocols
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