Recombinant human CD40 protein (Active) is a Human Fragment protein, in the 21 to 193 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, FuncS
- Biologically active
- Yes
Amino acid sequence
E P P T A C R E K Q Y L I N S Q C C S L C Q P G Q K L V S D C T E F T E T E C L P C G E S E F L D T W N R E T H C H Q H K Y C D P N L G L R V Q Q K G T S E T D T I C T C E E G W H C T S E A C E S C V L H R S C S P G F G V K Q I A T G V S D T I C E P C P V G F F S N V S S A F E K C H P W T S C E T K D L V V Q Q A G T N K T D V V C G P Q D R L R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Alternative names
CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Recommended products
Recombinant human CD40 protein (Active) is a Human Fragment protein, in the 21 to 193 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, FuncS
- Biologically active
- Yes
- Biological activity
- Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind ab155710 with a linear range of 0.039-1.25 μg/mL.
- Accession
- P25942-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris
Sequence info
Amino acid sequence
- E P P T A C R E K Q Y L I N S Q C C S L C Q P G Q K L V S D C T E F T E T E C L P C G E S E F L D T W N R E T H C H Q H K Y C D P N L G L R V Q Q K G T S E T D T I C T C E E G W H C T S E A C E S C V L H R S C S P G F G V K Q I A T G V S D T I C E P C P V G F F S N V S S A F E K C H P W T S C E T K D L V V Q Q A G T N K T D V V C G P Q D R L R
- Accession
- P25942
- Protein length
- Fragment
- Predicted molecular weight
- 45.3 kDa
- Amino acids
- 21 to 193
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
>90% as determined by SEC-HPLC.Protein A purified.
General info
- Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Measured by its binding ability in a functional ELISA. Immobilized ab155710 at 2 µg/ml (100 µl/well) can bind biotinylated Human CD40L with a linear range of 7.8 - 125 ng/ml.
Product promise
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3 product images
SDS-PAGE - Recombinant human CD40 protein (Active) (ab155710)
SDS-PAGE of reduced ab155710 stained overnight with Coomassie Blue.
ELISA - Recombinant human CD40 protein (Active) (ab155710)
Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind ab155710 with a linear range of 0.039-1.25 μg/mL.
Functional Studies - Recombinant human CD40 protein (Active) (ab155710)
ab155710 at 0.1 μg/ml can bind to 293T cells overexpressing human CD40L as determined by FACS analysis.
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