Recombinant human CD40 protein (Fc Chimera Active)

• FuncS

Unknown

Functional Studies - Recombinant human CD40 protein (Fc Chimera Active) (AB83917)

Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.

• WB

Unknown

Western blot - Recombinant human CD40 protein (Fc Chimera Active) (AB83917)

All lanes:

Western blot - Anti-CD40 antibody (<a href='/en-us/products/primary-antibodies/cd40-antibody-ab13545'>ab13545</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant human CD40 protein (Fc Chimera Active) (ab83917) at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 30 kDa

Observed band size: 72 kDa

true

Exposure time: 8min

• SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CD40 protein (Fc Chimera Active) (AB83917)

1D SDS-PAGE of ab83917 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1 : ab83917
Lane 2 : ab83917 treated with PNGase F to remove potential N-linked glycans
Lane 3 : ab83917 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.