Recombinant Human CD45 protein (Tagged) is a Human Fragment protein, in the 24 to 575 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
Q S P T P S P T G L T T A K M P S V P L S S D P L P T H T T A F S P A S T F E R E N D F S E T T T S L S P D N T S T Q V S P D S L D N A S A F N T T G V S S V Q T P H L P T H A D S Q T P S A G T D T Q T F S G S A A N A K L N P T P G S N A I S D V P G E R S T A S T F P T D P V S P L T T T L S L A H H S S A A L P A R T S N T T I T A N T S D A Y L N A S E T T T L S P S G S A V I S T T T I A T T P S K P T C D E K Y A N I T V D Y L Y N K E T K L F T A K L N V N E N V E C G N N T C T N N E V H N L T E C K N A S V S I S H N S C T A P D K T L I L D V P P G V E K F Q L H D C T Q V E K A D T T I C L K W K N I E T F T C D T Q N I T Y R F Q C G N M I F D N K E I K L E N L E P E H E Y K C D S E I L Y N N H K F T N A S K I I K T D F G S P G E P Q I I F C R S E A A H Q G V I T W N P P Q R S F H N F T L C Y I K E T E K D C L N L D K N L I K Y D L Q N L K P Y T K Y V L S L H A Y I I A K V Q R N G S A A M C H F T T K S A P P S Q V W N M T V S M T S D N S M H V K C R P P R D R N G P H E R Y H L E V E A G N T L V R N E S H K N C D F R V K D L Q Y S T D Y T F K A Y F H N G D Y P G E P F I L H H S T S Y N S K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).(Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Alternative names
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA, PTPRC, CD45
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Recombinant Human CD45 protein (Tagged) is a Human Fragment protein, in the 24 to 575 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P08575-3
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
Q S P T P S P T G L T T A K M P S V P L S S D P L P T H T T A F S P A S T F E R E N D F S E T T T S L S P D N T S T Q V S P D S L D N A S A F N T T G V S S V Q T P H L P T H A D S Q T P S A G T D T Q T F S G S A A N A K L N P T P G S N A I S D V P G E R S T A S T F P T D P V S P L T T T L S L A H H S S A A L P A R T S N T T I T A N T S D A Y L N A S E T T T L S P S G S A V I S T T T I A T T P S K P T C D E K Y A N I T V D Y L Y N K E T K L F T A K L N V N E N V E C G N N T C T N N E V H N L T E C K N A S V S I S H N S C T A P D K T L I L D V P P G V E K F Q L H D C T Q V E K A D T T I C L K W K N I E T F T C D T Q N I T Y R F Q C G N M I F D N K E I K L E N L E P E H E Y K C D S E I L Y N N H K F T N A S K I I K T D F G S P G E P Q I I F C R S E A A H Q G V I T W N P P Q R S F H N F T L C Y I K E T E K D C L N L D K N L I K Y D L Q N L K P Y T K Y V L S L H A Y I I A K V Q R N G S A A M C H F T T K S A P P S Q V W N M T V S M T S D N S M H V K C R P P R D R N G P H E R Y H L E V E A G N T L V R N E S H K N C D F R V K D L Q Y S T D Y T F K A Y F H N G D Y P G E P F I L H H S T S Y N S K
- Accession
- Protein length
Fragment
- Predicted molecular weight
147 kDa
- Amino acids
24 to 575
- Nature
Recombinant
Specifications
- Form
Liquid
General info
- Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
- Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
- Post-translational modifications
Heavily N- and O-glycosylated.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
- Biological function summary
CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.
- Pathways
Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.
- Associated diseases and disorders
Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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SDS-PAGE - Recombinant Human CD45 protein (Tagged) (ab236938)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab236938.
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