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Recombinant Human CD46 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.
Alternative names=Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, MIC10, CD46, MCP
- Purity
>=80% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
sELISA, SDS-PAGE
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.(Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.(Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.(Microbial infection) Acts as a receptor for cultured Measles virus.(Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.(Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes (PubMed:7708671, PubMed:9379894, PubMed:11260136, PubMed:11971006).
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Recombinant Human CD46 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.
Alternative names=Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, MIC10, CD46, MCP
Key facts
- Purity
>=80% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
sELISA, SDS-PAGE
- Biologically active
No
- Predicted molecular weight
30.5 kDa
- Accession
- P15529-1
- Protein length
Fragment
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Specifications
- Form
Liquid
General info
- Function
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
- Post-translational modifications
N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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