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Recombinant human CD47 protein (Active) is a Human Fragment protein, in the 19 to 139 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

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Images

Functional Studies - Recombinant human CD47 protein (Active) (AB220594), expandable thumbnail
  • SDS-PAGE - Recombinant human CD47 protein (Active) (AB220594), expandable thumbnail
  • Functional Studies - Recombinant human CD47 protein (Active) (AB220594), expandable thumbnail

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Tags
Avi tag C-Terminus His tag C-Terminus
Applications
SDS-PAGE, FuncS
Biologically active
Yes

Amino acid sequence

Q L L F N K T K S V E F T F C N D T V V I P C F V T N M E A Q N T T E V Y V K W K F K G R D I Y T F D G A L N K S T V P T D F S S A K I E V S Q L L K G D A S L K M D K S D A V S H T G N Y T C E V T E L T R E G E T I I E L K Y R V V S W F S P

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Adhesive protein that mediates cell-to-cell interactions (PubMed:11509594, PubMed:15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed:19004835, PubMed:7691831, PubMed:8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:11509594, PubMed:15383453, PubMed:19004835, PubMed:27742621, PubMed:32679764, PubMed:7691831, PubMed:8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed:27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed:11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed:15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed:32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed:7691831). May prevent premature elimination of red blood cells (By similarity).

Alternative names

Recommended products

Recombinant human CD47 protein (Active) is a Human Fragment protein, in the 19 to 139 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE, FuncS
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Human SIRP alpha, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 2-13 ng/ml.

Immobilized Anti-CD47 MAb, Human IgG4 at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 0.05-2.0 ng/ml.

Accession
Q08722-1
Animal free
No
Species
Human
Reconstitution
Reconstitute at 200 µg/mL in water
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10% Trehalose, 0.08% Bicine

Sequence info

Amino acid sequence

Q L L F N K T K S V E F T F C N D T V V I P C F V T N M E A Q N T T E V Y V K W K F K G R D I Y T F D G A L N K S T V P T D F S S A K I E V S Q L L K G D A S L K M D K S D A V S H T G N Y T C E V T E L T R E G E T I I E L K Y R V V S W F S P
Accession
Q08722
Protein length
Fragment
Predicted molecular weight
16.9 kDa
Amino acids
19 to 139
Nature
Recombinant
Tags
Avi tag C-Terminus, His tag C-Terminus

Specifications

Form
Lyophilized

General info

Function

Adhesive protein that mediates cell-to-cell interactions (PubMed:11509594, PubMed:15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed:19004835, PubMed:7691831, PubMed:8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:11509594, PubMed:15383453, PubMed:19004835, PubMed:27742621, PubMed:32679764, PubMed:7691831, PubMed:8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed:27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed:11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed:15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed:32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed:7691831). May prevent premature elimination of red blood cells (By similarity).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

After reconstitution this product is stable for 3 months at -80°C under sterile conditions.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD47 also referred to as integrin-associated protein carries a molecular weight of approximately 50 kDa. This protein is broadly expressed across various cell types notably on erythrocytes leukocytes and endothelial cells. Its transmembrane glycoprotein structure allows it to perform various cellular functions. CD47 interacts with specific ligands prominently SIRPα which are present on immune cells such as macrophages and dendritic cells. The engagement of CD47 with these ligands plays a major role in cellular signaling processes.

Biological function summary

CD47 functions as a "don't eat me" signal inhibiting phagocytosis by binding to SIRPα on macrophages. This protein is also important for cell adhesion and migration and can modulate interactions with integrins. While not a part of a large complex CD47 associates with various cytoskeletal and membrane proteins to maintain cellular architecture and transmission of mechanical forces. Additionally its interaction with thrombospondins influences angiogenesis and nitric oxide signaling.

Pathways

CD47 is extensively involved in the regulation of the immune response and angiogenesis. The interaction between CD47 and SIRPα plays a part in the regulation of macrophage activity impacting the immune signaling pathway. CD47 also interacts with integrins allowing it to contribute to the angiogenesis pathway which is critical in the formation of new blood vessels. These interactions help coordinate complex cellular responses and maintain tissue homeostasis.

Associated diseases and disorders

CD47 levels have associations with cancer and chronic inflammatory diseases. In cancer CD47 overexpression can allow tumor cells to evade the immune response by downregulating phagocytosis through SIRPα interaction. Therapeutically targeting CD47 using agents like anti-CD47 antibodies shows potential for enhancing anti-tumor immunity. Additionally CD47 is involved in atherosclerosis where its interaction with thrombospondin-1 contributes to disease pathogenesis by affecting nitric oxide signaling and vascular remodeling.

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3 product images

  • Functional Studies - Recombinant human CD47 protein (Active) (ab220594), expandable thumbnail

    Functional Studies - Recombinant human CD47 protein (Active) (ab220594)

    Immobilized Human SIRP alpha, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 2-13 ng/ml.

  • SDS-PAGE - Recombinant human CD47 protein (Active) (ab220594), expandable thumbnail

    SDS-PAGE - Recombinant human CD47 protein (Active) (ab220594)

    SDS-PAGE analysis of reduced ab220594 stained overnight with Coomassie Blue.

    The protein migrates as 33-44 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.

  • Functional Studies - Recombinant human CD47 protein (Active) (ab220594), expandable thumbnail

    Functional Studies - Recombinant human CD47 protein (Active) (ab220594)

    Immobilized Anti-CD47 MAb, Human IgG4 at 2 μg/ml (100 μl/well) can bind ab220594 with a linear range of 0.05-2.0 ng/ml.

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Product protocols

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