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AB220587

Recombinant human CD47 protein (Fc Chimera Active)

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Recombinant human CD47 protein (Fc Chimera Active) is a Human Fragment protein, in the 19 to 139 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Flow Cyt.

View Alternative Names

CD47, MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, IAP

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Biological Activity - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)
  • Biological Activity

Supplier Data

Biological Activity - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)

Loaded Recombinant human CD47 protein (Fc Chimera Active) (ab220587) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (ab174006) with an affinity constant of 1.1 μM as determined in BLI assay

Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)
  • FuncS

Supplier Data

Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)

Immobilized ab220587 at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, His Tag (HPLC-verified) with a linear range of 0.016-0.5 μg/mL.

Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)
  • FuncS

Unknown

Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)

The purity of Human CD47 (Fc Tag) (HPLC-verified) was greater than 90% as determined by SEC-HPLC.

Flow Cytometry - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)
  • Flow Cyt

Supplier Data

Flow Cytometry - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)

FACS assay shows that ab220587 can bind to ACHN cell expressing SIRP-a. The concentration of CD47 used is 10 μg/mL.

Biological Activity - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)
  • Biological Activity

Supplier Data

Biological Activity - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 10 μg/mL. IC50=8.39 μg/mL

ELISA - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)
  • ELISA

Supplier Data

ELISA - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag (HPLC-verified) (ab220587) : Biotinylated Human SIRP alpha, Fc,Avitag (ab246052) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 μg/mL

SDS-PAGE - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CD47 protein (Fc Chimera Active) (AB220587)

Human CD47 (Fc Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

Flow Cyt, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized ab220587 at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, His Tag with a linear range of 0.016-0.5 μg/mL.

Accession

Q08722

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 1 mg/mL in water

Storage buffer

pH: 7.4 Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris

storage-buffer

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Complementary Products

Primary Antibodies

AB218810

Anti-CD47 antibody [EPR21794]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [EPR21794] (AB218810)

  • 5
  • 1
Proteins & Peptides

AB179982

Recombinant Mouse IgG2a protein (Fc fragment)

Biological Activity - Recombinant Mouse IgG2a protein (Fc fragment) (AB179982)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Proteins & Peptides

AB316501

Recombinant Human CD3 Epsilon Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CD3 Epsilon Protein Standard (His tag) (AB316501)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"10 µg/mL", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP","proteinLength":"Fragment","predictedMolecularWeight":"40.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":139,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q08722","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD47 also referred to as integrin-associated protein carries a molecular weight of approximately 50 kDa. This protein is broadly expressed across various cell types notably on erythrocytes leukocytes and endothelial cells. Its transmembrane glycoprotein structure allows it to perform various cellular functions. CD47 interacts with specific ligands prominently SIRPα which are present on immune cells such as macrophages and dendritic cells. The engagement of CD47 with these ligands plays a major role in cellular signaling processes.
Biological function summary

CD47 functions as a "don't eat me" signal inhibiting phagocytosis by binding to SIRPα on macrophages. This protein is also important for cell adhesion and migration and can modulate interactions with integrins. While not a part of a large complex CD47 associates with various cytoskeletal and membrane proteins to maintain cellular architecture and transmission of mechanical forces. Additionally its interaction with thrombospondins influences angiogenesis and nitric oxide signaling.

Pathways

CD47 is extensively involved in the regulation of the immune response and angiogenesis. The interaction between CD47 and SIRPα plays a part in the regulation of macrophage activity impacting the immune signaling pathway. CD47 also interacts with integrins allowing it to contribute to the angiogenesis pathway which is critical in the formation of new blood vessels. These interactions help coordinate complex cellular responses and maintain tissue homeostasis.

CD47 levels have associations with cancer and chronic inflammatory diseases. In cancer CD47 overexpression can allow tumor cells to evade the immune response by downregulating phagocytosis through SIRPα interaction. Therapeutically targeting CD47 using agents like anti-CD47 antibodies shows potential for enhancing anti-tumor immunity. Additionally CD47 is involved in atherosclerosis where its interaction with thrombospondin-1 contributes to disease pathogenesis by affecting nitric oxide signaling and vascular remodeling.
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Specifications

Form

Lyophilized

Additional notes

>90% as determined by SEC-HPLC.

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General info

Function

Adhesive protein that mediates cell-to-cell interactions (PubMed : 11509594, PubMed : 15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed : 19004835, PubMed : 7691831, PubMed : 8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed : 11509594, PubMed : 15383453, PubMed : 19004835, PubMed : 27742621, PubMed : 32679764, PubMed : 7691831, PubMed : 8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed : 27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed : 11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed : 15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed : 32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed : 7691831). May prevent premature elimination of red blood cells (By similarity).

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Product protocols

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Target data

Adhesive protein that mediates cell-to-cell interactions (PubMed : 11509594, PubMed : 15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed : 19004835, PubMed : 7691831, PubMed : 8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed : 11509594, PubMed : 15383453, PubMed : 19004835, PubMed : 27742621, PubMed : 32679764, PubMed : 7691831, PubMed : 8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed : 27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed : 11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed : 15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed : 32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed : 7691831). May prevent premature elimination of red blood cells (By similarity).
See full target information CD47
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