Recombinant human CD47 protein (Fc Chimera Active) is a Human Fragment protein, in the 19 to 139 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, Flow Cyt.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS, Flow Cyt
- Biologically active
- Yes
Amino acid sequence
Q L L F N K T K S V E F T F C N D T V V I P C F V T N M E A Q N T T E V Y V K W K F K G R D I Y T F D G A L N K S T V P T D F S S A K I E V S Q L L K G D A S L K M D K S D A V S H T G N Y T C E V T E L T R E G E T I I E L K Y R V V S W F S P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 10 µg/mL | Notes - |
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Target data
Function
Adhesive protein that mediates cell-to-cell interactions (PubMed:11509594, PubMed:15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed:19004835, PubMed:7691831, PubMed:8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:11509594, PubMed:15383453, PubMed:19004835, PubMed:27742621, PubMed:32679764, PubMed:7691831, PubMed:8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed:27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed:11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed:15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed:32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed:7691831). May prevent premature elimination of red blood cells (By similarity).
Alternative names
CD47, MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, IAP
Recommended products
Recombinant human CD47 protein (Fc Chimera Active) is a Human Fragment protein, in the 19 to 139 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, Flow Cyt.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, FuncS, Flow Cyt
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized ab220587 at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, His Tag with a linear range of 0.016-0.5 μg/mL.
- Accession
- Q08722-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 1 mg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.61% Tris
Sequence info
Amino acid sequence
- Q L L F N K T K S V E F T F C N D T V V I P C F V T N M E A Q N T T E V Y V K W K F K G R D I Y T F D G A L N K S T V P T D F S S A K I E V S Q L L K G D A S L K M D K S D A V S H T G N Y T C E V T E L T R E G E T I I E L K Y R V V S W F S P
- Accession
- Q08722
- Protein length
- Fragment
- Predicted molecular weight
- 40.4 kDa
- Amino acids
- 19 to 139
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
>90% as determined by SEC-HPLC.
General info
- Function
Adhesive protein that mediates cell-to-cell interactions (PubMed:11509594, PubMed:15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed:19004835, PubMed:7691831, PubMed:8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:11509594, PubMed:15383453, PubMed:19004835, PubMed:27742621, PubMed:32679764, PubMed:7691831, PubMed:8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed:27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed:11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed:15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed:32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed:7691831). May prevent premature elimination of red blood cells (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD47 also referred to as integrin-associated protein carries a molecular weight of approximately 50 kDa. This protein is broadly expressed across various cell types notably on erythrocytes leukocytes and endothelial cells. Its transmembrane glycoprotein structure allows it to perform various cellular functions. CD47 interacts with specific ligands prominently SIRPα which are present on immune cells such as macrophages and dendritic cells. The engagement of CD47 with these ligands plays a major role in cellular signaling processes.
- Biological function summary
CD47 functions as a "don't eat me" signal inhibiting phagocytosis by binding to SIRPα on macrophages. This protein is also important for cell adhesion and migration and can modulate interactions with integrins. While not a part of a large complex CD47 associates with various cytoskeletal and membrane proteins to maintain cellular architecture and transmission of mechanical forces. Additionally its interaction with thrombospondins influences angiogenesis and nitric oxide signaling.
- Pathways
CD47 is extensively involved in the regulation of the immune response and angiogenesis. The interaction between CD47 and SIRPα plays a part in the regulation of macrophage activity impacting the immune signaling pathway. CD47 also interacts with integrins allowing it to contribute to the angiogenesis pathway which is critical in the formation of new blood vessels. These interactions help coordinate complex cellular responses and maintain tissue homeostasis.
- Associated diseases and disorders
CD47 levels have associations with cancer and chronic inflammatory diseases. In cancer CD47 overexpression can allow tumor cells to evade the immune response by downregulating phagocytosis through SIRPα interaction. Therapeutically targeting CD47 using agents like anti-CD47 antibodies shows potential for enhancing anti-tumor immunity. Additionally CD47 is involved in atherosclerosis where its interaction with thrombospondin-1 contributes to disease pathogenesis by affecting nitric oxide signaling and vascular remodeling.
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7 product images
Flow Cytometry - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
FACS assay shows that ab220587 can bind to ACHN cell expressing SIRP-a. The concentration of CD47 used is 10 μg/mL.
SDS-PAGE - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
Human CD47 (Fc Tag) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
Immobilized ab220587 at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, His Tag (HPLC-verified) with a linear range of 0.016-0.5 μg/mL.
ELISA - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag (HPLC-verified) (ab220587): Biotinylated Human SIRP alpha, Fc,Avitag (ab246052) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 μg/mL
Biological Activity - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
Loaded Recombinant human CD47 protein (Fc Chimera Active) (ab220587) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) (Recombinant Human SIRP alpha protein ab174006) with an affinity constant of 1.1 μM as determined in BLI assay
Functional Studies - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
The purity of Human CD47 (Fc Tag) (HPLC-verified) was greater than 90% as determined by SEC-HPLC.
Biological Activity - Recombinant human CD47 protein (Fc Chimera Active) (ab220587)
FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 10 μg/mL. IC50=8.39 μg/mL
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