Recombinant Human CD55 protein (His tag) is a Human Full Length protein, in the 35 to 353 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P D C G L P P D V P N A Q P A L E G R T S F P E D T V I T Y K C E E S F V K I P G E K D S V I C L K G S Q W D I E E F C N R S C E V P T R L N S A S L K Q P Y I T Q N Y F P V G T V V E Y E C R P G Y R R E P S L S P K L T C L Q N L K W S T A V E F C K K K S C P N P G E I R N G Q I D V P G G I L F G A T I S F S C N T G Y K L F G S T S S F C L I S G S S V Q W S D P L P E C R E I Y C P A P P Q I D N G I I Q G E R D H Y G Y R Q S V T Y A C N K G F T M I G E H S I Y C T V N N D E G E W S G P P P E C R G K S L T S K V P P T V Q K P T T V N V P T T E V S P T S Q K T T T K T T T P N A Q A T R S T P V S R T T K H F H E T T P N K G S G T T S H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829). (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable). (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
Alternative names
CD55, CR, DAF, Complement decay-accelerating factor
Recommended products
Recombinant Human CD55 protein (His tag) is a Human Full Length protein, in the 35 to 353 aa range, expressed in Baculovirus infected insect, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P08174-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P D C G L P P D V P N A Q P A L E G R T S F P E D T V I T Y K C E E S F V K I P G E K D S V I C L K G S Q W D I E E F C N R S C E V P T R L N S A S L K Q P Y I T Q N Y F P V G T V V E Y E C R P G Y R R E P S L S P K L T C L Q N L K W S T A V E F C K K K S C P N P G E I R N G Q I D V P G G I L F G A T I S F S C N T G Y K L F G S T S S F C L I S G S S V Q W S D P L P E C R E I Y C P A P P Q I D N G I I Q G E R D H Y G Y R Q S V T Y A C N K G F T M I G E H S I Y C T V N N D E G E W S G P P P E C R G K S L T S K V P P T V Q K P T T V N V P T T E V S P T S Q K T T T K T T T P N A Q A T R S T P V S R T T K H F H E T T P N K G S G T T S H H H H H H
- Accession
- P08174
- Protein length
- Full Length
- Predicted molecular weight
- 36 kDa
- Amino acids
- 35 to 353
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829).
- Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
- Post-translational modifications
The Ser/Thr-rich domain is heavily O-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD55 also known as Decay-Accelerating Factor (DAF) is a glycoprotein that regulates the complement cascade. This protein typically weighs around 70 kDa. It is expressed in many cells including epithelial and endothelial cells and is often present on the surface of blood cells. CD55 plays an important role in protecting cells against lysis by inhibiting the formation and stability of C3 and C5 convertases which ultimately prevent cell destruction by the complement system.
- Biological function summary
CD55 helps maintain immune homeostasis by preventing the complement-mediated damage to host tissues. It functions as a member of the regulators of complement activation (RCA) family. While not necessarily part of a larger protein complex its function is related to other regulatory proteins such as CD46 and CD59 which cooperate to regulate complement activity. The DAF protein's immunology function is instrumental in ensuring that self-cells are not inadvertently attacked by the body's immune system.
- Pathways
CD55 significantly impacts the complement pathway which is a central component of the innate immune system. It directly interacts with complement components C3 and C5 accelerating their decay and disassembly. Through this pathway CD55 has a working relationship with other complement regulatory proteins like Factor H which also aids in controlling complement activation on the cell surface.
- Associated diseases and disorders
A dysfunctional CD55 can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH the absence or insufficiency of CD55 (and CD59) on red blood cells leads to complement-mediated lysis causing hemolytic anemia. In aHUS impaired regulation of the complement system including abnormalities in CD55 function exacerbates symptoms. CD55's role is therefore important in preventing these complement-related disorders and preserving cellular integrity in the human body.
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1 product image
SDS-PAGE - Recombinant Human CD55 protein (His tag) (ab229358)
15% SDS-PAGE analysis of 3 μg ab229358.
MW: 40-57 kDa (SDS-PAGE under reducing conditions).
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