Recombinant Human CD59 protein
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(1 Publication)
Recombinant Human CD59 protein is a Human Full Length protein, in the 26 to 102 aa range, expressed in Escherichia coli, with >90%, 5 EU/µg endotoxin level, suitable for SDS-PAGE.
View Alternative Names
CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human CD59 protein (AB134887)
SDS PAGE analysis of ab134887 (lanes 4-6), using BSA as a reference protein (lanes 1-3).
Lane 1 : 10μg BSA
Lane 2 : 5μg BSA
Lane 3 : 1μg BSA
Lane 4 : 10μl ab134887
Lane 5 : 5μl ab134887
Lane 6 : 1μl ab134887
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human CD59 protein (AB134887)
SDS PAGE analysis of ab134887 (lanes 4-5), using BSA as a reference protein (lanes 1-3). 4-20% gradient gel was used.
Lane 1 : 10μl BSA
Lane 2 : 5μl BSA
Lane 3 : 1μl BSA
Lane 4 : 20μl ab134887 (Stored at -20°C for 180 days)
Lane 5 : 20μl ab134887 (Stored at -20°C, then shift to 4°C for 30 days)
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Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.
Pathways
CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.
Specifications
Form
Liquid
Additional notes
ab134887 was refolded using temperature shift inclusion body refolding technology and chromatographically purified.
General info
Function
Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation (PubMed : 11882685, PubMed : 1698710, PubMed : 2475111, PubMed : 2475570, PubMed : 2606909, PubMed : 9053451). Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (PubMed : 11882685, PubMed : 1698710, PubMed : 36797260).. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit complement membrane attack complex (MAC) assembly on cell membranes.
Post-translational modifications
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine. Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.. Glycated (PubMed:10805801). Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes (PubMed:10805801).
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 24: PubMed37239905
2023
Applications
Unspecified application
Species
Unspecified reactive species
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