Recombinant Human CD6/T12 protein (Fc Chimera) is a Human Fragment protein, in the 1 to 398 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M W L F F G I T G L L T A A L S G H P S P A P P D Q L N T S S A E S E L W E P G E R L P V R L T N G S S S C S G T V E V R L E A S W E P A C G A L W D S R A A E A V C R A L G C G G A E A A S Q L A P P T P E L P P P P A A G N T S V A A N A T L A G A P A L L C S G A E W R L C E V V E H A C R S D G R R A R V T C A E N R A L R L V D G G G A C A G R V E M L E H G E W G S V C D D T W D L E D A H V V C R Q L G C G W A V Q A L P G L H F T P G R G P I H R D Q V N C S G A E A Y L W D C P G L P G Q H Y C G H K E D A G A V C S E H Q S W R L T G G A D R C E G Q V E V H F R G V W N T V C D S E W Y P S E A K V L C Q S L G C G T A V E R P K G L P H S L S G R M Y Y S C N G E E L T L S N C S W R F N N S N L C S Q S L A A R V L C S A S R S L H N L S T P E V P A S V Q T V T I E S S V T V K I E N K E S R E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed:15048703, PubMed:15294938, PubMed:16352806, PubMed:16914752, PubMed:24584089, PubMed:24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed:15294938, PubMed:16352806, PubMed:16914752). Contributes to the formation and maturation of the immunological synapse (PubMed:15294938, PubMed:16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed:17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed:17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed:17601777).
Alternative names
CD6, T-cell differentiation antigen CD6, T12, TP120
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Recombinant Human CD6/T12 protein (Fc Chimera) is a Human Fragment protein, in the 1 to 398 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P30203-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M W L F F G I T G L L T A A L S G H P S P A P P D Q L N T S S A E S E L W E P G E R L P V R L T N G S S S C S G T V E V R L E A S W E P A C G A L W D S R A A E A V C R A L G C G G A E A A S Q L A P P T P E L P P P P A A G N T S V A A N A T L A G A P A L L C S G A E W R L C E V V E H A C R S D G R R A R V T C A E N R A L R L V D G G G A C A G R V E M L E H G E W G S V C D D T W D L E D A H V V C R Q L G C G W A V Q A L P G L H F T P G R G P I H R D Q V N C S G A E A Y L W D C P G L P G Q H Y C G H K E D A G A V C S E H Q S W R L T G G A D R C E G Q V E V H F R G V W N T V C D S E W Y P S E A K V L C Q S L G C G T A V E R P K G L P H S L S G R M Y Y S C N G E E L T L S N C S W R F N N S N L C S Q S L A A R V L C S A S R S L H N L S T P E V P A S V Q T V T I E S S V T V K I E N K E S R E
- Accession
- P30203
- Protein length
- Fragment
- Predicted molecular weight
- 67.3 kDa
- Amino acids
- 1 to 398
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed:15048703, PubMed:15294938, PubMed:16352806, PubMed:16914752, PubMed:24584089, PubMed:24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed:15294938, PubMed:16352806, PubMed:16914752). Contributes to the formation and maturation of the immunological synapse (PubMed:15294938, PubMed:16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed:17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed:17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed:17601777).
- Post-translational modifications
After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD6 also known as T12 is a glycoprotein expressed on T cells and some subsets of B cells. The protein has a molecular mass of approximately 105-130 kDa. CD6 is primarily located on the surface of thymocytes mature T lymphocytes and on some B cell lines. It plays an important role in the immunological synapse serving as a receptor that aids in cell-to-cell communication during immune responses. The CD6 protein is also detectable in immune tissues including the spleen and lymph nodes.
- Biological function summary
CD6 functions involve T cell maturation and proliferation. The protein is part of the lymphocyte activation complex contributing to the modulation of T cell receptor (TCR) signaling. It interacts with other cell surface proteins such as CD3 and CD28 to influence the response of T cells to antigens. CD6 potentially modulates the threshold for T cell activation favoring the adjustment of immune responses to various antigens.
- Pathways
CD6 participates in the signaling pathways that regulate adaptive immune responses. It is involved in the T cell receptor (TCR) signaling pathway essential for T cell activation and differentiation. CD6 interacts with proteins like LCK and ZAP-70 which are pivotal for the TCR signaling cascade. Additionally CD6's role in immune synapse formation connects it to the modulation of cytokine production and immune cell communication.
- Associated diseases and disorders
CD6 associates with autoimmune diseases and multiple sclerosis. The protein's involvement in T cell activation and immune regulation makes it a candidate for studies targeting autoimmune pathologies. Autoimmune disorders such as rheumatoid arthritis have shown links with CD6's activity as its deregulation can lead to inappropriate immune responses. Additionally CD6's interaction with proteins like CD166 has implications in the pathogenesis of these disorders indicating a potential target for therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human CD6/T12 protein (Fc Chimera) (ab276728)
SDS-PAGE analysis of ab276728
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