Recombinant Human CD62E protein is a Human Fragment protein, in the 22 to 556 aa range, expressed in CHO, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
>95% SDS-PAGE
< 1 EU/µg
CHO cells
Tag free
SDS-PAGE, FuncS
No
W S Y N T S T E A M T Y D E A S A Y C Q Q R Y T H L V A I Q N K E E I E Y L N S I L S Y S P S Y Y W I G I R K V N N V W V W V G T Q K P L T E E A K N W A P G E P N N R Q K D E D C V E I Y I K R E K D V G M W N D E R C S K K K L A L C Y T A A C T N T S C S G H G E C V E T I N N Y T C K C D P G F S G L K C E Q I V N C T A L E S P E H G S L V C S H P L G N F S Y N S S C S I S C D R G Y L P S S M E T M Q C M S S G E W S A P I P A C N V V E C D A V T N P A N G F V E C F Q N P G S F P W N T T C T F D C E E G F E L M G A Q S L Q C T S S G N W D N E K P T C K A V T C R A V R Q P Q N G S V R C S H S P A G E F T F K S S C N F T C E E G F M L Q G P A Q V E C T T Q G Q W T Q Q I P V C E A F Q C T A L S N P E R G Y M N C L P S A S G S F R Y G S S C E F S C E Q G F V L K G S K R L Q C G P T G E W D N E K P T C E A V R C D A V H Q P P K G L V R C A H S P I G E F T Y K S S C A F S C E E G F E L H G S T Q L E C T S Q G Q W T E E V P S C Q V V K C S S L A V P G K I N M S C S G E P V F G T V C K F A C P E G W T L N G S A A R T C G A T G H W S G L L P T C E A P T E S N I P
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Due to glycosylation, CD62E migrates at an apparent molecular weight of approximately 65-85 kDa by SDS-PAGE analysis under reducing conditions. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2, SELE, ELAM1
Recombinant Human CD62E protein is a Human Fragment protein, in the 22 to 556 aa range, expressed in CHO, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2, SELE, ELAM1
>95% SDS-PAGE
< 1 EU/µg
CHO cells
Tag free
SDS-PAGE, FuncS
No
No
Yes
Human
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Constituents: 0.164% Sodium phosphate
W S Y N T S T E A M T Y D E A S A Y C Q Q R Y T H L V A I Q N K E E I E Y L N S I L S Y S P S Y Y W I G I R K V N N V W V W V G T Q K P L T E E A K N W A P G E P N N R Q K D E D C V E I Y I K R E K D V G M W N D E R C S K K K L A L C Y T A A C T N T S C S G H G E C V E T I N N Y T C K C D P G F S G L K C E Q I V N C T A L E S P E H G S L V C S H P L G N F S Y N S S C S I S C D R G Y L P S S M E T M Q C M S S G E W S A P I P A C N V V E C D A V T N P A N G F V E C F Q N P G S F P W N T T C T F D C E E G F E L M G A Q S L Q C T S S G N W D N E K P T C K A V T C R A V R Q P Q N G S V R C S H S P A G E F T F K S S C N F T C E E G F M L Q G P A Q V E C T T Q G Q W T Q Q I P V C E A F Q C T A L S N P E R G Y M N C L P S A S G S F R Y G S S C E F S C E Q G F V L K G S K R L Q C G P T G E W D N E K P T C E A V R C D A V H Q P P K G L V R C A H S P I G E F T Y K S S C A F S C E E G F E L H G S T Q L E C T S Q G Q W T E E V P S C Q V V K C S S L A V P G K I N M S C S G E P V F G T V C K F A C P E G W T L N G S A A R T C G A T G H W S G L L P T C E A P T E S N I P
Fragment
58.6 kDa
22 to 556
Recombinant
Lyophilized
Purity : Greater than 95% by SDS-PAGE gel and HPLC analyses.Sterile filtered through a 0.2 micron filter.
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Belongs to the selectin/LECAM family.
Blue Ice
A few weeks
-20°C
Avoid freeze / thaw cycle
Measured by its ability to support adhesion of U937 cells, a Human hystiocytic lymphoma cell line.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
This supplementary information is collated from multiple sources and compiled automatically.
CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.
E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.
CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.
E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.
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