Recombinant Human CD64 protein is a Human Full Length protein, in the 1 to 374 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M W F L T T L L L W V P V D G Q V D T T K A V I T L Q P P W V S V F Q E E T V T L H C E V L H L P G S S S T Q W F L N G T A T Q T S T P S Y R I T S A S V N D S G E Y R C Q R G L S G R S D P I Q L E I H R G W L L L Q V S S R V F T E G E P L A L R C H A W K D K L V Y N V L Y Y R N G K A F K F F H W N S N L T I L K T N I S H N G T Y H C S G M G K H R Y T S A G I S V T V K E L F P A P V L N A S V T S P L L E G N L V T L S C E T K L L L Q R P G L Q L Y F S F Y M G S K T L R G R N T S S E Y Q I L T A R R E D S G L Y W C E A A T E D G N V L K R S P E L E L Q V L G L Q L P T P V W F H V L F Y L A V G I M F L V N T V L W V T I R K E L K R K K K W D L E I S L D S G H E K K V I S S L Q E D R H L E E E L K C Q E Q K E E Q L Q E G V H R K E P Q G A T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.
Alternative names
CD64, FCG1, FCGR1, IGFR1, FCGR1A, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, Fc-gamma RIA, FcRI, FcgammaRIa
Recommended products
Recombinant Human CD64 protein is a Human Full Length protein, in the 1 to 374 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P12314-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M W F L T T L L L W V P V D G Q V D T T K A V I T L Q P P W V S V F Q E E T V T L H C E V L H L P G S S S T Q W F L N G T A T Q T S T P S Y R I T S A S V N D S G E Y R C Q R G L S G R S D P I Q L E I H R G W L L L Q V S S R V F T E G E P L A L R C H A W K D K L V Y N V L Y Y R N G K A F K F F H W N S N L T I L K T N I S H N G T Y H C S G M G K H R Y T S A G I S V T V K E L F P A P V L N A S V T S P L L E G N L V T L S C E T K L L L Q R P G L Q L Y F S F Y M G S K T L R G R N T S S E Y Q I L T A R R E D S G L Y W C E A A T E D G N V L K R S P E L E L Q V L G L Q L P T P V W F H V L F Y L A V G I M F L V N T V L W V T I R K E L K R K K K W D L E I S L D S G H E K K V I S S L Q E D R H L E E E L K C Q E Q K E E Q L Q E G V H R K E P Q G A T
- Accession
- P12314
- Protein length
- Full Length
- Predicted molecular weight
- 67.14 kDa
- Amino acids
- 1 to 374
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.
- Sequence similarities
Belongs to the immunoglobulin superfamily. FCGR1 family.
- Post-translational modifications
Phosphorylated on serine residues.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD64 also known as Fc gamma receptor 1a (FCGR1A) is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). This receptor weighs approximately 72 kDa and is expressed on the surface of immune cells such as macrophages monocytes and activated neutrophils. CD64 protein plays an important role in immune responses by binding to antibodies and mediating processes such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Researchers often use anti-CD64 antibodies and CD64 markers to study its expression in various cellular contexts especially in flow cytometry.
- Biological function summary
CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.
- Pathways
CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.
- Associated diseases and disorders
CD64 expression correlates strongly with autoimmune disorders and inflammatory conditions. The receptor's role in orchestrating immune cell activation makes it relevant in diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis CD64 interacts with immune complexes intensifying inflammation through enhancement of phagocytic activity. In sepsis increased CD64 marker expression on neutrophils can serve as a diagnostic indicator. Related proteins like CD16 and CD32 also participate in these disorders by modulating immune complex handling and cellular activation.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human CD64 protein (ab114416)
ab114416 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com