Recombinant human CD66b protein (His tag) is a Human Fragment protein, in the 1 to 319 aa range, expressed in HEK 293, with >96% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >96% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M G P I S A P S C R W R I P W Q G L L L T A S L F T F W N P P T T A Q L T I E A V P S N A A E G K E V L L L V H N L P Q D P R G Y N W Y K G E T V D A N R R I I G Y V I S N Q Q I T P G P A Y S N R E T I Y P N A S L L M R N V T R N D T G S Y T L Q V I K L N L M S E E V T G Q F S V H P E T P K P S I S S N N S N P V E D K D A V A F T C E P E T Q N T T Y L W W V N G Q S L P V S P R L Q L S N G N R T L T L L S V T R N D V G P Y E C E I Q N P A S A N F S D P V T L N V L Y G P D A P T I S P S D T Y Y H A G V N L N L S C H A A S N P P S Q Y S W S V N G T F Q Q Y T Q K L F I P N I T T K N S G S Y A C H T T N S A T G R N R T T V R M I T V S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:11590190, PubMed:2022629, PubMed:8776764). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:11590190, PubMed:2022629, PubMed:8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764).
Alternative names
CD66b, CGM6, CEACAM8, Cell adhesion molecule CEACAM8, CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Non-specific cross-reacting antigen NCA-95, CEA cell adhesion molecule 8
Recommended products
Recombinant human CD66b protein (His tag) is a Human Fragment protein, in the 1 to 319 aa range, expressed in HEK 293, with >96% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >96% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized human CEACAM6-his at 10 μg/mL (100 μl/well) can bind biotinylated human CEACAM8-his. The EC50 of biotinylated human CEACAM8-his is 0.17 μg/mL.
- Accession
- P31997-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M G P I S A P S C R W R I P W Q G L L L T A S L F T F W N P P T T A Q L T I E A V P S N A A E G K E V L L L V H N L P Q D P R G Y N W Y K G E T V D A N R R I I G Y V I S N Q Q I T P G P A Y S N R E T I Y P N A S L L M R N V T R N D T G S Y T L Q V I K L N L M S E E V T G Q F S V H P E T P K P S I S S N N S N P V E D K D A V A F T C E P E T Q N T T Y L W W V N G Q S L P V S P R L Q L S N G N R T L T L L S V T R N D V G P Y E C E I Q N P A S A N F S D P V T L N V L Y G P D A P T I S P S D T Y Y H A G V N L N L S C H A A S N P P S Q Y S W S V N G T F Q Q Y T Q K L F I P N I T T K N S G S Y A C H T T N S A T G R N R T T V R M I T V S
- Accession
- P31997
- Protein length
- Fragment
- Predicted molecular weight
- 32.8 kDa
- Amino acids
- 1 to 319
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:11590190, PubMed:2022629, PubMed:8776764). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:11590190, PubMed:2022629, PubMed:8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764).
- Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
- Post-translational modifications
Glycosylated.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD66b also known as carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8) is a glycoprotein with a molecular weight around 95 kDa. It is primarily expressed on the surface of granulocytes specifically on neutrophils—its expression on these cells makes it a useful marker in distinguishing stages of granulocyte maturation. The protein localizes to the cell membrane and serves as a cell adhesion molecule playing roles in intercellular interactions. Researchers often use CD66b-specific antibodies such as CD66b FITC for detecting and analyzing neutrophil populations commonly in flow cytometry applications.
- Biological function summary
CD66b contributes to immune responses by facilitating leukocyte activation and migration during inflammation. It does not form part of a multi-protein complex but interacts with other cellular components. CD66b promotes neutrophil adhesion and transmigration to sites of infection or injury supporting their role in innate immunity. Additionally it participates in signaling pathways that influence cell survival proliferation and apoptosis in response to environmental cues.
- Pathways
CD66b operates within neutrophil-related processes like the immune response and cell adhesion pathways. In the immune response pathway it collaborates with integrins to assist neutrophil extravasation through the endothelium. During inflammatory responses CD66b interaction with integrins and other adhesion molecules influences the intensity and duration of activity at inflammation sites. This interaction highlights its involvement with proteins such as ICAM-1 in mediating firm adhesion necessary for neutrophil migration.
- Associated diseases and disorders
CD66b levels and activity correlate with conditions involving inflammation such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. During COPD the altered expression and regulation of CD66b contribute to neutrophil-driven inflammation exacerbating disease symptoms. Similarly rheumatoid arthritis involves inflammatory pathways where aberrant CD66b activity enhances synovial inflammation often linked with other inflammatory markers like TNF-alpha. Understanding CD66b's role in such diseases aids the development of therapeutic strategies targeting neutrophil-mediated pathology.
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2 product images
Functional Studies - Recombinant human CD66b protein (His tag) (ab276425)
Measured by its binding ability in a functional ELISA. Immobilized human CEACAM6-his at 10 μg/mL (100 μl/well) can bind biotinylated human CEACAM8-his. The EC50 of biotinylated human CEACAM8-his is 0.17 μg/mL.
SDS-PAGE - Recombinant human CD66b protein (His tag) (ab276425)
SDS-PAGE analysis of ab276425
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