Recombinant Human CD70 protein (Tagged) (Biotin) is a Human Fragment protein, in the 39 to 193 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
Amino acid sequence
Q R F A Q A Q Q Q L P L E S L G W D V A E L Q L N H T G P Q Q D P R L Y W Q G G P A L G R S F L H G P E L D K G Q L R I H R D G I Y M V H I Q V T L A I C S S T T A S R H H P T T L A V G I C S P A S R S I S L L R L S F H Q G C T I A S Q R L T P L A R G D T L C T N L T G T L L P S R N T D E T F F G V Q W V R P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864).
Alternative names
CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27-L
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Recombinant Human CD70 protein (Tagged) (Biotin) is a Human Fragment protein, in the 39 to 193 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Conjugation
- Biotin
- Accession
- P32970-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- Q R F A Q A Q Q Q L P L E S L G W D V A E L Q L N H T G P Q Q D P R L Y W Q G G P A L G R S F L H G P E L D K G Q L R I H R D G I Y M V H I Q V T L A I C S S T T A S R H H P T T L A V G I C S P A S R S I S L L R L S F H Q G C T I A S Q R L T P L A R G D T L C T N L T G T L L P S R N T D E T F F G V Q W V R P
- Accession
- P32970
- Protein length
- Fragment
- Predicted molecular weight
- 20 kDa
- Amino acids
- 39 to 193
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864).
- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Enzymatically biotin-labeled using Avi-tag™ technology
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD70 also known as CD27 ligand or CD27L is a type II transmembrane protein with a molecular mass of approximately 30 kDa. It belongs to the tumor necrosis factor (TNF) superfamily and is encoded by the CD70 gene. The expression of CD70 occurs mainly on activated T and B cells dendritic cells and some NK cells but not on resting lymphocytes. In addition CD70 expression is observed in certain malignancies and inflammatory conditions.
- Biological function summary
CD70 plays an essential role in the regulation of immune responses. It does this by binding to its receptor CD27 forming a receptor-ligand complex that triggers signaling pathways vital for T cell proliferation survival and differentiation. This interaction also influences the production of immunoglobulins by B cells. CD70-CD27 signaling is particularly important in shaping adaptive immunity enhancing the body's ability to respond to pathogens effectively.
- Pathways
CD70 is integral in the activation of the NF-κB and MAPK pathways. These pathways are activated upon the interaction of CD70 with CD27 promoting cell survival proliferation and pro-inflammatory cytokine production. Other proteins related to CD70 through these pathways include TRAF2 and TRAF3 which are essential for downstream signaling events that modulate immune responses.
- Associated diseases and disorders
CD70 has been implicated in autoimmune diseases and certain cancers. For instance it plays a role in the pathogenesis of lupus by contributing to abnormal immune activation. Overexpression of CD70 is also associated with various cancers such as renal cell carcinoma where it can support tumor cell growth and immune evasion. Through these conditions CD70 interacts with proteins like BCL-2 in cancer affecting cell survival pathways while its interaction with CD27 influences the immune deregulation seen in lupus.
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SDS-PAGE - Recombinant Human CD70 protein (Tagged) (Biotin) (ab271443)
SDS-PAGE analysis of 4 μg ab271443.
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