Recombinant human CD80 protein (Active) is a Human Fragment protein, in the 35 to 242 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, HPLC, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, HPLC, FuncS
- Biologically active
- Yes
Amino acid sequence
V I H V T K E V K E V A T L S C G H N V S V E E L A Q T R I Y W Q K E K K M V L T M M S G D M N I W P E Y K N R T I F D I T N N L S I V I L A L R P S D E G T Y E C V V L K Y E K D A F K R E H L A E V T L S V K A D F P T P S I S D F E I P T S N I R R I I C S T S G G F P E P H L S W L E N G E E L N A I N T T V S Q D P E T E L Y A V S S K L D F N M T T N H S F M C L I K Y G H L R V N Q T F N W N T T K Q E H F P D N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed:38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed:10438913). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed:12121659). Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed:36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity). (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Alternative names
CD80, CD28LG, CD28LG1, LAB7, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7
Recommended products
Recombinant human CD80 protein (Active) is a Human Fragment protein, in the 35 to 242 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, HPLC, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, HPLC, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (ab246118) with a linear range of 0.02-1.25 μg/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Recombinant human CTLA4 protein (ab167727) with a linear range of 0.5-4 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 (biotinylated) protein (ab207136) with a linear range of 0.6-10 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse CD28, His, Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind ab173993 with a linear range of 0.2-2 ng/mL.
Measured by its binding ability in a SPR assay. Immobilized Human CD28, on CM5 Chip can bind ab173993 with an affinity constant of 2.01 μM.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag with a linear range of 0.1-2 ng/mL (QC tested).
- Accession
- P33681-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% Trehalose, 0.75% Glycine, 0.61% Tris
Sequence info
Amino acid sequence
- V I H V T K E V K E V A T L S C G H N V S V E E L A Q T R I Y W Q K E K K M V L T M M S G D M N I W P E Y K N R T I F D I T N N L S I V I L A L R P S D E G T Y E C V V L K Y E K D A F K R E H L A E V T L S V K A D F P T P S I S D F E I P T S N I R R I I C S T S G G F P E P H L S W L E N G E E L N A I N T T V S Q D P E T E L Y A V S S K L D F N M T T N H S F M C L I K Y G H L R V N Q T F N W N T T K Q E H F P D N
- Accession
- P33681
- Protein length
- Fragment
- Predicted molecular weight
- 50 kDa
- Amino acids
- 35 to 242
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed:38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed:10438913). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed:12121659). Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed:36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).
- Post-translational modifications
Palmitoylated by ZDHHC20; palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
B7-1 and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T and Bcell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B71 and B72 with a 20 100 fold higher affinity than CD28 and is involved in the downregulation of the immune response.
B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.
- Biological function summary
CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.
- Pathways
CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.
- Associated diseases and disorders
CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.
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8 product images
SDS-PAGE - Recombinant human CD80 protein (Active) (ab173993)
Reduced SDS-PAGE analysis of ab173993, stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
ELISA - Recombinant human CD80 protein (Active) (ab173993)
Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 (biotinylated) protein (ab207136) with a linear range of 0.6-10 ng/mL.
Functional Studies - Recombinant human CD80 protein (Active) (ab173993)
FACS assay shows that ab173993 can bind to 293T cells overexpressing human CTLA4 protein. The concentration of ab173993 is 1 μg/mL.
Functional Studies - Recombinant human CD80 protein (Active) (ab173993)
Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (ab246118) with a linear range of 0.02-1.25 μg/mL
Functional Studies - Recombinant human CD80 protein (Active) (ab173993)
Immobilized Human CD28, on CM5 Chip can bind ab173993 with an affinity constant of 2.01 μM as determined in a SPR assay.
ELISA - Recombinant human CD80 protein (Active) (ab173993)
Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Recombinant human CTLA4 protein (Recombinant human CTLA4 protein (Active) ab167727) with a linear range of 0.5-4 ng/mL.
ELISA - Recombinant human CD80 protein (Active) (ab173993)
Immobilized Biotinylated Mouse CD28, His, Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind ab173993 with a linear range of 0.2-2 ng/mL.
ELISA - Recombinant human CD80 protein (Active) (ab173993)
ab173993 at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag with a linear range of 0.1-2 ng/mL (QC tested).
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