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Recombinant human CD80 protein (Active) is a Human Fragment protein, in the 35 to 242 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, HPLC, FuncS.
Alternative names=T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7, CD28LG, CD80, CD28LG1, LAB7
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag C-Terminus
- Applications
SDS-PAGE, ELISA, HPLC, FuncS
- Biologically active
Yes
- Sequence
V I H V T K E V K E V A T L S C G H N V S V E E L A Q T R I Y W Q K E K K M V L T M M S G D M N I W P E Y K N R T I F D I T N N L S I V I L A L R P S D E G T Y E C V V L K Y E K D A F K R E H L A E V T L S V K A D F P T P S I S D F E I P T
S N I R R I I C S T S G G F P E P H L S W L E N G E E L N A I N T T V S Q D P E T E L Y A V S S K L D F N M T T N H S F
M C L I K Y G H L R V N Q T F N W N T T K Q E H F P D N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human CD80 protein (Active) is a Human Fragment protein, in the 35 to 242 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, HPLC, FuncS.
Alternative names=T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7, CD28LG, CD80, CD28LG1, LAB7
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag C-Terminus
- Applications
SDS-PAGE, ELISA, HPLC, FuncS
- Biologically active
Yes
- Biological activity
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, His Tag, Fc Tag (ab246118) with a linear range of 0.02-1.25 μg/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Recombinant human CTLA4 protein (ab167727) with a linear range of 0.5-4 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 (biotinylated) protein (ab207136) with a linear range of 0.6-10 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse CD28, His, Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind ab173993 with a linear range of 0.2-2 ng/mL.
Measured by its binding ability in a SPR assay. Immobilized Human CD28, on CM5 Chip can bind ab173993 with an affinity constant of 2.01 μM.
Measured by its binding ability in a functional ELISA. Immobilized ab173993 at 2 μg/mL (100 μL/well) can bind Human CTLA-4, His Tag with a linear range of 0.1-2 ng/mL (QC tested).
- Predicted molecular weight
50 kDa
- Amino acids
35 to 242
- Sequence
V I H V T K E V K E V A T L S C G H N V S V E E L A Q T R I Y W Q K E K K M V L T M M S G D M N I W P E Y K N R T I F D I T N N L S I V I L A L R P S D E G T Y E C V V L K Y E K D A F K R E H L A E V T L S V K A D F P T P S I S D F E I P T
S N I R R I I C S T S G G F P E P H L S W L E N G E E L N A I N T T V S Q D P E T E L Y A V S S K L D F N M T T N H S F
M C L I K Y G H L R V N Q T F N W N T T K Q E H F P D N- Accession
- P33681-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% Trehalose, 0.75% Glycine, 0.61% Tris
Specifications
- Form
Lyophilized
General info
- Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
B7-1 and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T and Bcell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B71 and B72 with a 20 100 fold higher affinity than CD28 and is involved in the downregulation of the immune response.
B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
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