Recombinant human CD86 protein (Fc Chimera) is a Human Fragment protein, in the 26 to 247 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
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- Molecular neurobiology 58:1074-10872020Neuroprotective Effect of Fractalkine on Radiation-induced Brain Injury Through Promoting the M2 Polarization of Microglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaojiao Wang,Huijiao Pan,Zhenyu Lin,Chunjin Xiong,Chunhua Wei,Huanhuan Li,Fan Tong,Xiaorong DongPubMed 33089423
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Alternative names
CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
Recommended products
Recombinant human CD86 protein (Fc Chimera) is a Human Fragment protein, in the 26 to 247 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its ability to induce IL2 secretion by Jurkat Human acute T cell leukemia cells. The ED50 for this effect is typically 0.04 - 0.2 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human ab167720 with a linear range of 2-78 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind ab167720 with a linear range of 5-78 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized ab167720 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.
- Accession
- P42081-1
- Animal free
- No
- Species
- Human
- Reconstitution
- It is recommended to reconstitute the lyophilized protein with 500 µL sterile deionized water to a stock solution of 400 µg/mL.
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 0.75% Glycine, 0.48% Tris
Sequence info
Amino acid sequence
- Accession
- P42081
- Protein length
- Fragment
- Predicted molecular weight
- 51.5 kDa
- Amino acids
- 26 to 247
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).
- Post-translational modifications
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
- Biological function summary
CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.
- Pathways
CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.
- Associated diseases and disorders
CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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7 product images
SDS-PAGE - Recombinant human CD86 protein (Fc Chimera) (ab167720)
Human B7-2, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The protein migrates as 66-86 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).
Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720)
FACS assay shows that Human B7-2, Fc Tag can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-2 is 3 µg/ml.
Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720)
FACS analysis shows that the binding of Human B7-2, Fc Tag to CD28 expressed on Jurkat E6.1 was inhibited by increasing concentration of neutralizing anti-CD28 antibody. The concentration of B7-2 used is 2 μg/ml. The IC50 is 0.022 μg/ml.
Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720)
Flow Cytometry assay shows that recombinant Human B7-2, Fc Tag can bind to CD28 expressed on Jurkat E6.1. The concentration of B7-2 used is 2 µg/ml(Routinely tested).
ELISA - Recombinant human CD86 protein (Fc Chimera) (ab167720)
Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human ab167720 with a linear range of 2-78 ng/mL.
ELISA - Recombinant human CD86 protein (Fc Chimera) (ab167720)
Immobilized Biotinylated Mouse CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind ab167720 with a linear range of 5-78 ng/mL.
Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720)
Immobilized ab167720 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.
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