Skip to main content

Recombinant human CD86 protein (Fc Chimera) is a Human Fragment protein, in the 26 to 247 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

Be the first to review this product! Submit a review

Images

SDS-PAGE - Recombinant human CD86 protein (Fc Chimera) (AB167720), expandable thumbnail
  • Functional Studies - Recombinant human CD86 protein (Fc Chimera) (AB167720), expandable thumbnail
  • Functional Studies - Recombinant human CD86 protein (Fc Chimera) (AB167720), expandable thumbnail
  • Functional Studies - Recombinant human CD86 protein (Fc Chimera) (AB167720), expandable thumbnail
  • ELISA - Recombinant human CD86 protein (Fc Chimera) (AB167720), expandable thumbnail

Publications

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Tags
Fc tag C-Terminus
Applications
SDS-PAGE, ELISA, FuncS
Biologically active
Yes

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

Alternative names

Recommended products

Recombinant human CD86 protein (Fc Chimera) is a Human Fragment protein, in the 26 to 247 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Applications
SDS-PAGE, ELISA, FuncS
Biological activity
Measured by its ability to induce IL2 secretion by Jurkat Human acute T cell leukemia cells. The ED50 for this effect is typically 0.04 - 0.2 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human ab167720 with a linear range of 2-78 ng/mL.

Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Mouse CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind ab167720 with a linear range of 5-78 ng/mL.

Measured by its binding ability in a functional ELISA. Immobilized ab167720 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.

Accession
P42081-1
Animal free
No
Species
Human
Reconstitution
It is recommended to reconstitute the lyophilized protein with 500 µL sterile deionized water to a stock solution of 400 µg/mL.
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: L-Arginine, Sodium chloride, 0.75% Glycine, 0.48% Tris

Sequence info

Amino acid sequence

Accession
P42081
Protein length
Fragment
Predicted molecular weight
51.5 kDa
Amino acids
26 to 247
Nature
Recombinant
Tags
Fc tag C-Terminus

Specifications

Form
Lyophilized

General info

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).

Post-translational modifications

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.

Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

Associated diseases and disorders

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • SDS-PAGE - Recombinant human CD86 protein (Fc Chimera) (ab167720), expandable thumbnail

    SDS-PAGE - Recombinant human CD86 protein (Fc Chimera) (ab167720)

    Human B7-2, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. The protein migrates as 66-86 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720), expandable thumbnail

    Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720)

    FACS assay shows that Human B7-2, Fc Tag can bind to 293T cells overexpressing human CTLA4 protein. The concentration of Human B7-2 is 3 µg/ml.

  • Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720), expandable thumbnail

    Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720)

    FACS analysis shows that the binding of Human B7-2, Fc Tag to CD28 expressed on Jurkat E6.1 was inhibited by increasing concentration of neutralizing anti-CD28 antibody. The concentration of B7-2 used is 2 μg/ml. The IC50 is 0.022 μg/ml.

  • Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720), expandable thumbnail

    Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720)

    Flow Cytometry assay shows that recombinant Human B7-2, Fc Tag can bind to CD28 expressed on Jurkat E6.1. The concentration of B7-2 used is 2 µg/ml(Routinely tested).

  • ELISA - Recombinant human CD86 protein (Fc Chimera) (ab167720), expandable thumbnail

    ELISA - Recombinant human CD86 protein (Fc Chimera) (ab167720)

    Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human ab167720 with a linear range of 2-78 ng/mL.

  • ELISA - Recombinant human CD86 protein (Fc Chimera) (ab167720), expandable thumbnail

    ELISA - Recombinant human CD86 protein (Fc Chimera) (ab167720)

    Immobilized Biotinylated Mouse CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind ab167720 with a linear range of 5-78 ng/mL.

  • Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720), expandable thumbnail

    Functional Studies - Recombinant human CD86 protein (Fc Chimera) (ab167720)

    Immobilized ab167720 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc Tag, Avi Tag with a linear range of 0.6-10 ng/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com