JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB307475

Recombinant Human CD86 protein (Fc Chimera)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human CD86 protein (Fc Chimera) is a Human Fragment protein, in the 24 to 247 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE.

View Alternative Names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

3 Images
Mass Spectrometry - Recombinant Human CD86 protein (Fc Chimera) (AB307475)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human CD86 protein (Fc Chimera) (AB307475)

Mass determination by ESI-TOF. Predicted MW is 51489.45 Da (+/-10 Da by ESI-TOF). Observed MW is 51370.39 Da.

HPLC - Recombinant Human CD86 protein (Fc Chimera) (AB307475)
  • HPLC

Supplier Data

HPLC - Recombinant Human CD86 protein (Fc Chimera) (AB307475)

HPLC analysis of ab307475

SDS-PAGE - Recombinant Human CD86 protein (Fc Chimera) (AB307475)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CD86 protein (Fc Chimera) (AB307475)

SDS-PAGE analysis of ab307475

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE, HPLC, Mass Spec

applications

Biologically active

No

Accession

P42081

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB288813

Recombinant Human CD80 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human CD80 protein (Active) (AB288813)

  • 0
  • 0
Primary Antibodies

AB239075

Anti-CD86 antibody [EPR21962]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-CD86 antibody [EPR21962] (AB239075)

  • 0
  • 0
Proteins & Peptides

AB322096

Recombinant Human SIRP alpha Protein Standard (His tag)

Sandwich ELISA - Recombinant Human SIRP alpha Protein Standard (His tag) (AB322096)

  • 0
  • 0
Primary Antibodies

AB15323

Anti-iNOS antibody

BOND RX™ Validated

Western blot - Anti-iNOS antibody (AB15323)

  • 4
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"APLKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP","proteinLength":"Fragment","predictedMolecularWeight":"51.49 kDa","actualMolecularWeight":"51.37 kDa","aminoAcidEnd":247,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P42081","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
style
left-column

Specifications

Form

Lyophilized

Additional notes

SDS-PAGE >= 95%

style
left-column

General info

Function

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 12196291, PubMed : 7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291, PubMed : 7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 11279501).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

Post-translational modifications

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

style
left-column

Product protocols

style
left-column

Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 12196291, PubMed : 7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291, PubMed : 7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 11279501).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD86
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com