Recombinant Human CD86 protein (Fc Chimera) is a Human Fragment protein, in the 24 to 247 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
Amino acid sequence
A P L K I Q A Y F N E T A D L P C Q F A N S Q N Q S L S E L V V F W Q D Q E N L V L N E V Y L G K E K F D S V H S K Y M G R T S F D S D S W T L R L H N L Q I K D K G L Y Q C I I H H K K P T G M I R I H Q M N S E L S V L A N F S Q P E I V P I S N I T E N V Y I N L T C S S I H G Y P E P K K M S V L L R T K N S T I E Y D G V M Q K S Q D N V T E L Y D V S I S L S V S F P D V T S N M T I F C I L E T D K T R L L S S P F S I E L E D P Q P P P D H I P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Alternative names
CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
Recommended products
Recombinant Human CD86 protein (Fc Chimera) is a Human Fragment protein, in the 24 to 247 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
- Accession
- P42081-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A P L K I Q A Y F N E T A D L P C Q F A N S Q N Q S L S E L V V F W Q D Q E N L V L N E V Y L G K E K F D S V H S K Y M G R T S F D S D S W T L R L H N L Q I K D K G L Y Q C I I H H K K P T G M I R I H Q M N S E L S V L A N F S Q P E I V P I S N I T E N V Y I N L T C S S I H G Y P E P K K M S V L L R T K N S T I E Y D G V M Q K S Q D N V T E L Y D V S I S L S V S F P D V T S N M T I F C I L E T D K T R L L S S P F S I E L E D P Q P P P D H I P
- Accession
- P42081
- Protein length
- Fragment
- Predicted molecular weight
- 51.49 kDa
- Actual molecular weight
- 51.37 kDa
- Amino acids
- 24 to 247
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).
- Post-translational modifications
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
- Biological function summary
CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.
- Pathways
CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.
- Associated diseases and disorders
CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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3 product images
Mass Spectrometry - Recombinant Human CD86 protein (Fc Chimera) (ab307475)
Mass determination by ESI-TOF. Predicted MW is 51489.45 Da (+/-10 Da by ESI-TOF). Observed MW is 51370.39 Da.
SDS-PAGE - Recombinant Human CD86 protein (Fc Chimera) (ab307475)
SDS-PAGE analysis of ab307475
HPLC - Recombinant Human CD86 protein (Fc Chimera) (ab307475)
HPLC analysis of ab307475
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