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Recombinant Human CD86 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.

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Images

Sandwich ELISA - Recombinant Human CD86 Protein Standard (His tag) (AB316708), expandable thumbnail
  • SDS-PAGE - Recombinant Human CD86 Protein Standard (His tag) (AB316708), expandable thumbnail

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

Biologically active

No

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).Isoform 2Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.(Microbial infection) Acts as a receptor for adenovirus subgroup B.

Alternative names

Recombinant Human CD86 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.

Key facts

Purity

>=80% SDS-PAGE

Expression system

HEK 293 cells

Applications

SDS-PAGE, sELISA

Accession
P42081-1
Animal free

Yes

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Accession

P42081

Protein length

Fragment

Predicted molecular weight

27.1 kDa

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

General info

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed:12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).

Post-translational modifications

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.

Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

Associated diseases and disorders

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Sandwich ELISA - Recombinant Human CD86 Protein Standard (His tag) (ab316708), expandable thumbnail

    Sandwich ELISA - Recombinant Human CD86 Protein Standard (His tag) (ab316708)

    Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

  • SDS-PAGE - Recombinant Human CD86 Protein Standard (His tag) (ab316708), expandable thumbnail

    SDS-PAGE - Recombinant Human CD86 Protein Standard (His tag) (ab316708)

    SDS-PAGE analysis of ab316708 under reducing conditions for 2ug protein.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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