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AB105911

Recombinant human Cdc25C protein

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Recombinant human Cdc25C protein is a Human Full Length protein, in the 1 to 473 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C

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Functional Studies - Recombinant human Cdc25C protein (AB105911)
  • FuncS

Unknown

Functional Studies - Recombinant human Cdc25C protein (AB105911)

The specific activity of Cdc25C (ab105911) was determined to be 22 phosphate released/min/mg as per activity assay protocol

Functional Studies - Recombinant human Cdc25C protein (AB105911)
  • FuncS

Unknown

Functional Studies - Recombinant human Cdc25C protein (AB105911)

Phosphatase Assay demonstrating specific activity of ab105911.

SDS-PAGE - Recombinant human Cdc25C protein (AB105911)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Cdc25C protein (AB105911)

SDS PAGE analysis of ab105911

SDS-PAGE - Recombinant human Cdc25C protein (AB105911)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Cdc25C protein (AB105911)

SDS-PAGE showing ab105911 at approximately 84kDa.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The Specific activity of ab105911 was determined to be 22 nmol/min/mg.

Accession

P30307

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

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Complementary Products

Primary Antibodies

AB32444

Anti-Cdc25C antibody [E302]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C antibody [E302] (AB32444)

  • 4
  • 3
Proteins & Peptides

AB128559

Recombinant human PPP1A/PPP1CA protein

Functional Studies - Recombinant human PPP1A/PPP1CA protein (AB128559)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB207327

Anti-Albumin antibody [EPR20195]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"84 kDa","actualMolecularWeight":null,"aminoAcidEnd":473,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P30307","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc25C also known as dual specificity phosphatase Cdc25C is a 65-kDa protein that acts as a cell cycle regulator controlling the transition from the G2 to M phase. The protein is expressed in various tissues but has higher expression levels in actively dividing cells. Cdc25C is important for cell division due to its ability to activate cyclin-dependent kinases (CDKs) by dephosphorylating the inactive phosphorylated forms. This action makes Cdc25C an important target for regulating the cell cycle and ensuring proper cell proliferation.
Biological function summary

The proper function of Cdc25C involves facilitating proper cell cycle progression by coordinating with other cell cycle regulators. It participates in a complex network where it dephosphorylates and activates CDK1/cyclin B1 complexes promoting the mitotic entry. This function is important for maintaining genomic stability during cell division. Misregulation of Cdc25C can lead to cell cycle arrest or uncontrolled cell proliferation highlighting its essential role in cell cycle control mechanisms.

Pathways

Cdc25C fits into the cell cycle checkpoint pathways and is also a part of the DNA damage response pathways. It connects with the p53 and ATM/ATR signaling proteins while responding to DNA damage ensuring a temporary pause in cell cycle progression for repair mechanisms to act. Proper interaction with these pathways is essential for maintaining cellular integrity and preventing the proliferation of damaged cells with CDK1 and Wee1 kinase serving as major interacting proteins in these processes.

Misregulation or overexpression of Cdc25C can relate to cancer and various cell proliferation disorders. In cancer Cdc25C's disordered expression often links to unchecked cell division and tumor progression commonly involving proteins such as p53 which act as tumor suppressors. Furthermore abnormalities in Cdc25C function may lead to issues related to cell cycle progression errors implicating it in other proliferative disorders such as hyperplasia where it acts in conjunction with and alters the activities of proteins like cyclin B1.
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >70% by densitometry. Affinity purified.

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General info

Function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed : 8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed : 8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed : 8119945).

Sequence similarities

Belongs to the MPI phosphatase family.

Post-translational modifications

Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

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Product protocols

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Target data

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed : 8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed : 8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed : 8119945).
See full target information CDC25C
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