Recombinant Human Cdc26 / Apc12 protein is a Human Full Length protein, in the 1 to 85 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M L R R K P T R L E L K L D D I E E F E N I R K D L E T R K K Q K E D V E V V G G S D G E G A I G L S S D P K S R E Q M I N D R I G Y K P Q P K P N N R S S Q F G S L E F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132). May recruit the E2 ubiquitin-conjugating enzymes to the complex (PubMed:18485873).
Alternative names
ANAPC12, C9orf17, CDC26, Anaphase-promoting complex subunit CDC26, Anaphase-promoting complex subunit 12, Cell division cycle protein 26 homolog, APC12
Recommended products
Recombinant Human Cdc26 / Apc12 protein is a Human Full Length protein, in the 1 to 85 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q8NHZ8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M L R R K P T R L E L K L D D I E E F E N I R K D L E T R K K Q K E D V E V V G G S D G E G A I G L S S D P K S R E Q M I N D R I G Y K P Q P K P N N R S S Q F G S L E F
- Accession
- Q8NHZ8
- Protein length
- Full Length
- Predicted molecular weight
- 11.9 kDa
- Amino acids
- 1 to 85
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab108123 is purified using conventional chromatography techniques.
General info
- Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132). May recruit the E2 ubiquitin-conjugating enzymes to the complex (PubMed:18485873).
- Sequence similarities
Belongs to the CDC26 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cdc26 also known as Apc12 is a protein that plays a mechanical role as a subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C). This protein complex is essential for cell cycle regulation especially during the transition from metaphase to anaphase. Cdc26 has a mass of approximately 90 kDa. Expression of Cdc26 occurs mainly in dividing cells indicating its involvement in processes requiring tight cell cycle control.
- Biological function summary
Cdc26/Apc12 functions as a component of the APC/C which is important for ubiquitin-mediated proteolysis of cell cycle proteins. As part of this large multi-subunit complex it aids the degradation of securin and cyclins which are proteins needed for cell cycle progression. The activity of Cdc26 is necessary to ensure correct chromosome separation during mitosis making it fundamental for maintaining genomic stability.
- Pathways
Cdc26 integrates into cell cycle regulatory pathways particularly in the mitotic exit and G1 maintenance. It collaborates closely with other proteins such as Cdc20 and Cdh1 which together regulate the activity of the APC/C complex. These interactions highlight its role within the ubiquitin-proteasome signaling pathway and highlight its importance in maintaining orderly progression through the cell cycle.
- Associated diseases and disorders
Cdc26 is implicated in cancer due to its critical function in cell cycle regulation and maintenance of genomic integrity. Aberrant expression or malfunction of Cdc26 may lead to uncontrolled cell division contributing to tumorigenesis. Other proteins related to Cdc26 in cancer pathways like Cyclin B1 also play significant roles in uncontrolled cell proliferation. Additionally Cdc26 has associations with genetic disorders affecting cell division further underlining its significance in healthy cellular function.
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1 product image
SDS-PAGE - Recombinant Human Cdc26 / Apc12 protein (ab108123)
15% SDS-PAGE analysis of 3µg ab108123.
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