Recombinant Human Cdc34 protein is a Human Full Length protein, in the 1 to 236 aa range, expressed in Escherichia coli, with >75% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M A R P L V P S S Q K A L L L E L K G L Q E E P V E G F R V T L V D E G D L Y N W E V A I F G P P N T Y Y E G G Y F K A R L K F P I D Y P Y S P P A F R F L T K M W H P N I Y E T G D V C I S I L H P P V D D P Q S G E L P S E R W N P T Q N V R T I L L S V I S L L N E P N T F S P A N V D A S V M Y R K W K E S K G K D R E Y T D I I R K Q V L G T K V D A E R D G V K V P T T L A E Y C V K T K A P A P D E G S D L F Y D D Y Y E D G E V E E E A D S C F G D D E D D S G T E E S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (PubMed:10329681, PubMed:17588522, PubMed:20061386, PubMed:38326650). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:10329681, PubMed:10918611, PubMed:17698585). Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1 (PubMed:10871850, PubMed:15652359, PubMed:19112177). Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation (PubMed:19126550). Also involved in the degradation of beta-catenin (PubMed:12037680). Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:11805320, PubMed:12060736).
Alternative names
UBCH3, UBE2R1, CDC34, Ubiquitin-conjugating enzyme E2 R1, (E3-independent) E2 ubiquitin-conjugating enzyme R1, E2 ubiquitin-conjugating enzyme R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1
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Recombinant Human Cdc34 protein is a Human Full Length protein, in the 1 to 236 aa range, expressed in Escherichia coli, with >75% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P49427-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.76% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M A R P L V P S S Q K A L L L E L K G L Q E E P V E G F R V T L V D E G D L Y N W E V A I F G P P N T Y Y E G G Y F K A R L K F P I D Y P Y S P P A F R F L T K M W H P N I Y E T G D V C I S I L H P P V D D P Q S G E L P S E R W N P T Q N V R T I L L S V I S L L N E P N T F S P A N V D A S V M Y R K W K E S K G K D R E Y T D I I R K Q V L G T K V D A E R D G V K V P T T L A E Y C V K T K A P A P D E G S D L F Y D D Y Y E D G E V E E E A D S C F G D D E D D S G T E E S
- Accession
- P49427
- Protein length
- Full Length
- Predicted molecular weight
- 33 kDa
- Amino acids
- 1 to 236
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
assessed by densitometry
General info
- Function
E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (PubMed:10329681, PubMed:17588522, PubMed:20061386, PubMed:38326650). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:10329681, PubMed:10918611, PubMed:17698585). Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1 (PubMed:10871850, PubMed:15652359, PubMed:19112177). Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation (PubMed:19126550). Also involved in the degradation of beta-catenin (PubMed:12037680). Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:11805320, PubMed:12060736).
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
- Post-translational modifications
Autoubiquitinated (PubMed:11805320, PubMed:12060736, PubMed:22496338). Autoubiquitination is promoted by the human herpes virus 1 protein ICP0 and leads to degradation by the Ubiquitin-proteasomal pathway (PubMed:11805320, PubMed:12060736).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Cdc34 protein also known as Ubc3 is a ubiquitin-conjugating enzyme with a molecular mass of about 35 kDa. It is expressed in various tissues with high presence in actively dividing cells. Cdc34 plays a significant role in cell cycle regulation particularly during the transition from G1 to the S phase. It facilitates the attachment of ubiquitin molecules to target proteins marking them for degradation by the proteasome which is important for controlling the abundance of specific proteins involved in cell cycle progression.
- Biological function summary
Cdc34 functions as a member of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex. This complex targets a range of key regulatory proteins for ubiquitination influencing processes such as DNA replication and repair. By mediating protein degradation Cdc34 helps regulate the cellular levels of cyclins which are essential for proper cell cycle advancement. Its activity ensures timely degradation of these cyclins thereby preventing unscheduled cell cycle transitions that can lead to cellular dysfunction.
- Pathways
Cdc34 is integrated in the ubiquitin-proteasome pathway and the cell cycle pathway. In these contexts Cdc34's activity closely interacts with proteins like Skp2 another component of the SCF complex which regulates G1-S phase transition. Cdc34-related pathways ensure that protein turnover is tightly controlled which is essential for maintaining cellular homeostasis and responding to internal and external signals during cell proliferation.
- Associated diseases and disorders
Cdc34 links to cancer and neurodegenerative disorders. Alterations in Cdc34 function can result in the aberrant degradation of regulatory proteins contributing to unchecked cell proliferation a hallmark of cancer. Furthermore imbalances in ubiquitin-proteasome pathway components including Cdc34 relate to neurodegenerative diseases where protein aggregation occurs. Connections to proteins such as p27 which regulates cell cycle and accumulation issues in neurological tissues highlight the significance of Cdc34 in disease mechanisms.
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SDS-PAGE - Recombinant Human Cdc34 protein (ab151828)
SDS-PAGE analysis of ab151828 which was determined to be >75% pure by densitometry.
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