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AB177571

Recombinant Human CDK11B protein (GST tag N-Terminus)

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Recombinant Human CDK11B protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 795 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE.

View Alternative Names

CDC2L1, CDK11, PITSLREA, CDK11B, Cyclin-dependent kinase 11B, Cell division cycle 2-like protein kinase 1, Cell division protein kinase 11B, Galactosyltransferase-associated protein kinase p58/GTA, PITSLRE serine/threonine-protein kinase CDC2L1, p58 CLK-1, CLK-1

1 Images
SDS-PAGE - Recombinant Human CDK11B protein (AB177571)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CDK11B protein (AB177571)

SDS-PAGE analysis of ab177571.

Key facts

Purity

>70% Densitometry

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB198813

Anti-CDK11A/CDK11B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK11A/CDK11B Antibody (AB198813)

  • 0
  • 0
Primary Antibodies

AB7778

Anti-Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

  • 4
  • 24
Primary Antibodies

AB306587

Anti-CXCL10/IP-10 antibody [EPR24674-84]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL10/IP-10 antibody [EPR24674-84] (AB306587)

  • 0
  • 0
Primary Antibodies

AB236640

Anti-Collagen IV antibody [EPR22911-127]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Collagen IV antibody [EPR22911-127] (AB236640)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHRMEITIRNSPYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKDEKRKEKRRHRSHSAEGGKHARVKEKEREHERRKRHREEQDKARREWERQKRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMREDYSDKVKASHWSRSPPRPPRERFELGDGRKPGEARPARAQKPAQLKEEKMEERDLLSDLQDISDSERKTSSAESSSAESGSGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEERENENHLLVVPESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALSPIELKQELPKYLPALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVVVTLWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEIDQINKVFKDLGTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLKF","proteinLength":"Full Length","predictedMolecularWeight":"135 kDa","actualMolecularWeight":null,"aminoAcidEnd":795,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P21127","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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General info

Function

Cyclin-dependent protein kinase that acts as a regulator of transcription and pre-mRNA splicing (PubMed : 12501247, PubMed : 18216018, PubMed : 32367068, PubMed : 36104565). Acts as a key regulator of pre-mRNA splicing by mediating phosphorylation of SF3B1, enabling the association between SF3B1 and U5 and U6 snRNAs in the activated spliceosome, thereby promoting spliceosome assembly (PubMed : 36104565, PubMed : 38059508). Also acts as a regulator of transcription by phosphorylating 'Ser-2' of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 32367068, PubMed : 40858114). Involved in replication-dependent transcription of histone genes : binds to histone genes and phosphorylates POLR2A at 'Ser-2' of the CTD to specifically control transcriptional elongation of histones and recruitment of 3'-end processing factors (PubMed : 32367068). Part of a transcription checkpoint upstream of CDK9, which regulates promoter-proximal pausing by RNA polymerase II, a transcription halt following transcription initiation, but prior to elongation (PubMed : 40858114). Probably regulates promoter-proximal pausing by mediating phosphorylation of POLR2A at 'Ser-2' of the CTD (PubMed : 40858114).. Isoform SV9. Isoform expressed in a non-cell cycle-dependent manner.. Isoform 7. Isoform specifically expressed during the G2-M phases of the cell cycle (PubMed : 12082095, PubMed : 2217177). Phosphorylates 'Ser-2' of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 38019613). Promotes centromeric transcription to maintain centromeric cohesion during mitosis (PubMed : 24436071, PubMed : 38019613).

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Post-translational modifications

Isoform SV9. During FAS- or TNF-induced apoptosis, isoform SV9 is cleaved by caspases to produce p110C, a fragment that contains the C-terminal kinase domain.. Phosphorylation at Ser-115 creates a binding site for 14-3-3 proteins. p110C can be autophosphorylated.

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Product protocols

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Target data

Cyclin-dependent protein kinase that acts as a regulator of transcription and pre-mRNA splicing (PubMed : 12501247, PubMed : 18216018, PubMed : 32367068, PubMed : 36104565). Acts as a key regulator of pre-mRNA splicing by mediating phosphorylation of SF3B1, enabling the association between SF3B1 and U5 and U6 snRNAs in the activated spliceosome, thereby promoting spliceosome assembly (PubMed : 36104565, PubMed : 38059508). Also acts as a regulator of transcription by phosphorylating 'Ser-2' of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 32367068, PubMed : 40858114). Involved in replication-dependent transcription of histone genes : binds to histone genes and phosphorylates POLR2A at 'Ser-2' of the CTD to specifically control transcriptional elongation of histones and recruitment of 3'-end processing factors (PubMed : 32367068). Part of a transcription checkpoint upstream of CDK9, which regulates promoter-proximal pausing by RNA polymerase II, a transcription halt following transcription initiation, but prior to elongation (PubMed : 40858114). Probably regulates promoter-proximal pausing by mediating phosphorylation of POLR2A at 'Ser-2' of the CTD (PubMed : 40858114).. Isoform SV9. Isoform expressed in a non-cell cycle-dependent manner.. Isoform 7. Isoform specifically expressed during the G2-M phases of the cell cycle (PubMed : 12082095, PubMed : 2217177). Phosphorylates 'Ser-2' of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed : 38019613). Promotes centromeric transcription to maintain centromeric cohesion during mitosis (PubMed : 24436071, PubMed : 38019613).
See full target information CDK11B
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