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AB85639

Recombinant human CDK2 + CCNE1 protein

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(3 Publications)

Recombinant human CDK2 + CCNE1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

CCNE, CCNE1, G1/S-specific cyclin-E1

4 Images
Functional Studies - Recombinant human CDK2 + CCNE1 protein (AB85639)
  • FuncS

Unknown

Functional Studies - Recombinant human CDK2 + CCNE1 protein (AB85639)

The specific activity of CDK2 + CCNE1 (ab85639) was determined to be 345 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human CDK2 + CCNE1 protein (AB85639)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CDK2 + CCNE1 protein (AB85639)

SDS PAGE analysis of ab85639

Functional Studies - Recombinant human CDK2 + CCNE1 protein (AB85639)
  • FuncS

Unknown

Functional Studies - Recombinant human CDK2 + CCNE1 protein (AB85639)

The Specific activity of ab85639 was determined to be 404 nmol/min/mg.

SDS-PAGE - Recombinant human CDK2 + CCNE1 protein (AB85639)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CDK2 + CCNE1 protein (AB85639)

SDS-PAGE showing ab85639 at approximately 58kDa (CDK2) and 73kDa (CCNE1).

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

The Specific activity of ab85639 was determined to be 404 nmol/min/mg.

Accession

P24864

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P24941","tags":[{"tag":"GST","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P24864","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >90% by densitometry. Affinity purified.

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General info

Function

Essential for the control of the cell cycle at the G1/S (start) transition.

Sequence similarities

Belongs to the cyclin family. Cyclin E subfamily.

Post-translational modifications

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the control of the cell cycle at the G1/S (start) transition.
See full target information CCNE1

Additional targets

CDK2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioorganic & medicinal chemistry 26:3491-3501 PubMed29853338

2018

Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yiting Wang,Yanmei Chen,Xiaoling Cheng,Ke Zhang,Hangyu Wang,Bo Liu,Jinhui Wang

Developmental cell 41:274-286.e5 PubMed28457793

2017

Multi-site Neurogenin3 Phosphorylation Controls Pancreatic Endocrine Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Azzarelli,Christopher Hurley,Magdalena K Sznurkowska,Steffen Rulands,Laura Hardwick,Ivonne Gamper,Fahad Ali,Laura McCracken,Christopher Hindley,Fiona McDuff,Sonia Nestorowa,Richard Kemp,Kenneth Jones,Berthold Göttgens,Meritxell Huch,Gerard Evan,Benjamin D Simons,Douglas Winton,Anna Philpott

Molecular and cellular biology 34:2732-44 PubMed24820417

2014

Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoko Kitagawa,Kiyoshi Shibata,Akinobu Matsumoto,Masaki Matsumoto,Tatsuya Ohhata,Keiichi I Nakayama,Hiroyuki Niida,Masatoshi Kitagawa
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Proteins & Peptides

AB85836

Recombinant Human CDK2 + Cyclin E1 protein

proteins-peptides

recombinant-human-cdk2-cyclin-e1-protein-ab85836

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(0 reviews)

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