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AB85836

Recombinant Human CDK2 + Cyclin E1 protein

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Recombinant Human CDK2 + Cyclin E1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

CCNE, CCNE1, G1/S-specific cyclin-E1

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SDS-PAGE - Recombinant Human CDK2 + Cyclin E1 protein (AB85836)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human CDK2 + Cyclin E1 protein (AB85836)

SDS-PAGE showing ab85836 at approximately 58 kDa (CDK2) and 73 kDa (CCNE1).

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P24864

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"73 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P24864","tags":[{"tag":"GST","terminus":"N-Terminus"}]},{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"58 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P24941","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Essential for the control of the cell cycle at the G1/S (start) transition.

Sequence similarities

Belongs to the cyclin family. Cyclin E subfamily.

Post-translational modifications

Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Essential for the control of the cell cycle at the G1/S (start) transition.
See full target information CCNE1

Additional targets

CDK2
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Alternative Products

Proteins & Peptides

AB85639

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Secondary Antibodies

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Primary Antibodies

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Product promise

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