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AB85643

Recombinant human CDK3 + CCNE1 protein

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Recombinant human CDK3 + CCNE1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

CDKN3, CDK3, Cyclin-dependent kinase 3, Cell division protein kinase 3

4 Images
Functional Studies - Recombinant human CDK3 + CCNE1 protein (AB85643)
  • FuncS

Unknown

Functional Studies - Recombinant human CDK3 + CCNE1 protein (AB85643)

The specific activity of CDK3 + CCNE1 (ab85643) was determined to be 23 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human CDK3 + CCNE1 protein (AB85643)
  • FuncS

Unknown

Functional Studies - Recombinant human CDK3 + CCNE1 protein (AB85643)

The Specific activity of ab85643 was determined to be 20 nmol/min/mg.

SDS-PAGE - Recombinant human CDK3 + CCNE1 protein (AB85643)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CDK3 + CCNE1 protein (AB85643)

SDS-PAGE showing ab85643 at approximately 60kDa (CDK3) and 73kDa (CCNE1).

SDS-PAGE - Recombinant human CDK3 + CCNE1 protein (AB85643)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CDK3 + CCNE1 protein (AB85643)

SDS PAGE analysis of ab85643

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

The Specific activity of ab85643 was determined to be 20 nmol/min/mg.

Accession

Q00526

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase Assay</p>" } } }
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Product details

ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q00526","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purity was determined to be >90% by densitometry. Affinity purified.

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General info

Function

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

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Product protocols

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Target data

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.
See full target information CDK3

Additional targets

CCNE1
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Complementary Products

Primary Antibodies

AB33911

Anti-Cyclin E1 antibody [EP435E]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin E1 antibody [EP435E] (AB33911)

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Proteins & Peptides

AB111646

Recombinant Human CDK2 protein

SDS-PAGE - Recombinant Human CDK2 protein (AB111646)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB32147

Anti-Cdk2 antibody [E304]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Cdk2 antibody [E304] (AB32147)

  • 5
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Product promise

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