Recombinant Human Cdk4 protein (denatured) is a Human Full Length protein, in the 1 to 303 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A T S R Y E P V A E I G V G A Y G T V Y K A R D P H S G H F V A L K S V R V P N G G G G G G G L P I S T V R E V A L L R R L E A F E H P N V V R L M D V C A T S R T D R E I K V T L V F E H V D Q D L R T Y L D K A P P P G L P A E T I K D L M R Q F L R G L D F L H A N C I V H R D L K P E N I L V T S G G T V K L A D F G L A R I Y S Y Q M A L T P V V V T L W Y R A P E V L L Q S T Y A T P V D M W S V G C I F A E M F R R K P L F C G N S E A D Q L G K I F D L I G L P P E D D W P R D V S L P R G A F P P R G P R P V Q S V V P E M E E S G A Q L L L E M L T F N P H K R I S A F R A L Q H S Y L H K D E G N P E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Alternative names
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4
Recommended products
Recombinant Human Cdk4 protein (denatured) is a Human Full Length protein, in the 1 to 303 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Alternative names
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P11802-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A T S R Y E P V A E I G V G A Y G T V Y K A R D P H S G H F V A L K S V R V P N G G G G G G G L P I S T V R E V A L L R R L E A F E H P N V V R L M D V C A T S R T D R E I K V T L V F E H V D Q D L R T Y L D K A P P P G L P A E T I K D L M R Q F L R G L D F L H A N C I V H R D L K P E N I L V T S G G T V K L A D F G L A R I Y S Y Q M A L T P V V V T L W Y R A P E V L L Q S T Y A T P V D M W S V G C I F A E M F R R K P L F C G N S E A D Q L G K I F D L I G L P P E D D W P R D V S L P R G A F P P R G P R P V Q S V V P E M E E S G A Q L L L E M L T F N P H K R I S A F R A L Q H S Y L H K D E G N P E
- Accession
- Protein length
Full Length
- Predicted molecular weight
36.1 kDa
- Amino acids
1 to 303
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
- Post-translational modifications
Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.
- Subcellular localisation
Nucleus, Nucleus membrane
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cdk4 also known as Cyclin-dependent kinase 4 is a serine/threonine kinase involved in cell cycle regulation. Cdk4 has a molecular weight of approximately 34 kDa. This protein mainly locates in the nucleus of the cell and its expression occurs in diverse tissues. Cdk4 partners with D-type cyclins to exert its function which is essential for the transition from the G1 phase to the S phase of the cell cycle. Researchers often study Cdk4 using techniques like immunohistochemistry (IHC) to determine its expression and localization in different tissues.
- Biological function summary
Cdk4 plays a fundamental role in cell cycle regulation by forming a complex with D-type cyclins. Together they phosphorylate the retinoblastoma protein (Rb) resulting in the release of E2F transcription factors which promote the expression of genes necessary for DNA replication. The Cdk4/cyclin D complex regulates the cell's commitment to enter S phase and continue cell division. This regulation is key for normal cell proliferation and tissue homeostasis. Cdk4 activity is strictly controlled by INK4 family members acting as inhibitors and adding an extra regulation layer.
- Pathways
Cdk4 is an integral part of important signaling pathways like the cell cycle and PI3K/AKT pathways. In the cell cycle pathway Cdk4 relays signals downstream that drive the transition from the G1 to S phase through its interaction with cyclin D and Rb. In the PI3K/AKT pathway signaling can influence cyclin D expression indirectly affecting Cdk4 activity. Proteins such as Cdk6 closely relate to Cdk4 and often compensate or partner with Cdk4 in these pathways to maintain proper cell cycle progression.
- Associated diseases and disorders
Abnormalities in Cdk4 function or regulation relate to cancer and certain metabolic syndromes. Hyperactivity of Cdk4 due to overexpression or mutation frequently occurs in several cancers including melanoma and breast cancer. In these malignancies Cdk4 aberrantly drives excessive cell proliferation. Mutations or alterations in proteins such as p16 an inhibitor of Cdk4 are often found in these cancers highlighting the critical role of Cdk4 in disease progression. Understanding the role of Cdk4 allows researchers to develop targeted therapies such as Cdk4/6 inhibitors offering new treatment options.
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1 product image
SDS-PAGE - Recombinant Human Cdk4 protein (denatured) (ab134542)
15% SDS-PAGE analysis of 3 μg ab134542.
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