Recombinant Human CDK5R1(p25) protein. is a Human Full Length protein, in the 98 to 307 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, WB.
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- Experimental and therapeutic medicine 23:1472022Role of cyclin-dependent kinase 5 in early brain injury following experimental subarachnoid hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Ding,Liexiang Zhang,Wei Zhou,Hai Lu,Xingde Gao,Jian Li,Jingde Liu,Xiaowang Niu,Jing ZhengPubMed 35069828
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution.
Alternative names
CDK5R, NCK5A, CDK5R1, Cyclin-dependent kinase 5 activator 1, CDK5 activator 1, Cyclin-dependent kinase 5 regulatory subunit 1, TPKII regulatory subunit
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Recombinant Human CDK5R1(p25) protein. is a Human Full Length protein, in the 98 to 307 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- Q15078-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- Q15078
- Protein length
- Full Length
- Predicted molecular weight
- 49 kDa
- Amino acids
- 98 to 307
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity was determined to be >85% by densitometry. Affinity purified.
General info
- Function
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution.
- Sequence similarities
Belongs to the cyclin-dependent kinase 5 activator family.
- Post-translational modifications
The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo (By similarity).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human CDK5R1(p25) protein. (ab125653)
SDS-PAGE analysis of ab125653.
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