Recombinant Human Cdk6 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB.
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- Oncology reports 45:706-7162021miRNA‑490‑3p promotes the metastatic progression of invasive ductal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Lu,Mei Zhang,Lu Lu,Yan-Zhao Liu,Hai-Hong Zhang,Xiao-Dong LiuPubMed 33416185
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, WB
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).
Alternative names
Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6
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Recombinant Human Cdk6 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB.
Alternative names
Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, WB
- Biologically active
No
- Accession
- Q00534-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- Accession
- Protein length
Full Length
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Purity >90% as determined by densitometry. Affinity purified.
General info
- Function
Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).
- Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
- Post-translational modifications
Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.
- Subcellular localisation
Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cdk6 also known as Cyclin-dependent kinase 6 is a protein kinase involved in the regulation of the cell cycle. It has a molecular weight of approximately 40 kDa. Cdk6 is expressed in various tissues including lymphoid tissues and the brain. It plays a mechanical role by phosphorylating target proteins which impacts cell cycle progression particularly during the transition from G1 phase to S phase. Cdk6 can work in conjunction with D-type cyclins to form a complex that is critical for its activity.
- Biological function summary
Cdk6 functions as a regulator of the cell cycle. It participates in a complex with cyclin D to control the G1 phase progression. This activity is essential for the regulation of cell division and proliferation. The association with cyclin D allows Cdk6 to phosphorylate the retinoblastoma protein (Rb) leading to the release of E2F transcription factors that promote the expression of genes necessary for DNA synthesis.
- Pathways
Cdk6 is an important component of the cell cycle control pathway. It interacts primarily with the retinoblastoma protein and Cyclin D1 in this context. The cell cycle pathway is critical for controlled cell proliferation and its dysregulation can lead to diseases like cancer. Another involved pathway is the PI3K/AKT pathway which can activate Cdk6 activity through upstream signaling events implicating Cdk6 in cellular responses to growth signals.
- Associated diseases and disorders
Cdk6 has a significant role in cancer development due to its involvement in uncontrolled cell proliferation. Its overexpression or dysregulation is often linked to cancers such as leukemia and glioblastoma. In cancer the interaction with proteins like cyclin D1 can lead to unchecked progression through the cell cycle contributing to oncogenesis. Additionally Cdk6 inhibitors are being explored as therapeutic agents in cancer treatment due to their potential to restore control over cell cycle progression.
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1 product image
SDS-PAGE - Recombinant Human Cdk6 protein (ab84717)
SDS-PAGE of ab84717. Approximate MWt 39 kDa.
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