Skip to main content

Recombinant human CDK7 + Cyclin H + MNAT1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.

Be the first to review this product! Submit a review

Images

Functional Studies - Recombinant human CDK7 + Cyclin H + MNAT1 protein (AB64303), expandable thumbnail
  • SDS-PAGE - Recombinant human CDK7 + Cyclin H + MNAT1 protein (AB64303), expandable thumbnail
  • Functional Studies - Recombinant human CDK7 + Cyclin H + MNAT1 protein (AB64303), expandable thumbnail
  • SDS-PAGE - Recombinant human CDK7 + Cyclin H + MNAT1 protein (AB64303), expandable thumbnail

Publications

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

Enzyme activity.

Target data

Function

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts (PubMed:9852112). Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

Targets

Cyclin-H, CDK-activating kinase assembly factor MAT1

Alternative names

Recommended products

Recombinant human CDK7 + Cyclin H + MNAT1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

Specific activity: 19 nmol/min/mg.

Accession
P50613-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

Sequence info

Amino acid sequence

Accession

P50613

Protein length

Full Length

Nature

Recombinant

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts (PubMed:9852112). Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Post-translational modifications

Phosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity. Phosphorylated at Ser-164 and Thr-170 by CDK2.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Native Pig Myelin Basic Protein ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303), expandable thumbnail

    Functional Studies - Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303)

    The specific activity of CDK7 + Cyclin H + MNAT1 (ab64303) was determined to be 20 nmol/min/mg as per activity assay protocol

  • SDS-PAGE - Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303), expandable thumbnail

    SDS-PAGE - Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303)

    SDS PAGE analysis of ab64303

  • Functional Studies - Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303), expandable thumbnail

    Functional Studies - Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303)

    The specific activity of CDK7 + Cyclin H + MNAT1 (ab64303) was determined to be 19nmol/min/mg as per activity assay protocol.

  • SDS-PAGE - Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303), expandable thumbnail

    SDS-PAGE - Recombinant human CDK7 + Cyclin H + MNAT1 protein (ab64303)

    SDS-PAGE analysis of ab64303. The purity of CDK7 + Cyclin H + MNAT1 (ab64303) was determined to be >90% by densitometry, CDK7 approx. MW 40kDa, CyclinH1 approx. MW 39kDa and MNAT1 approx. MW 37kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com