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AB84075

Recombinant Human CDKN2A/p16INK4a protein

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(2 Publications)

Recombinant Human CDKN2A/p16INK4a protein is a Human Full Length protein, in the 1 to 156 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

CDKN2, MTS1, CDKN2A, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, p16-INK4a, CDK4I, MTS-1, p16-INK4, p16INK4A

1 Images
Sandwich ELISA - Recombinant Human CDKN2A/p16INK4a protein (AB84075)
  • sELISA

Unknown

Sandwich ELISA - Recombinant Human CDKN2A/p16INK4a protein (AB84075)

Standard Curve for CDKN2A/p16INK4a (Analyte : CDKN2A/p16INK4a protein (Human) (ab84075)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a (ab16123) at 1μg/ml and Detector Antibody Rabbit polyclonal to CDKN2A/p16INK4a (ab7962) at 0.5μg/ml.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P42771

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute with 1x PBS, pH 8.0. to a concentration of 0.1-1.0 mg/ml.

Storage buffer

Constituents: PBS, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Primary Antibodies

AB108349

Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal (AB108349)

  • 4
  • 7
Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

  • 4
  • 16
Proteins & Peptides

AB169843

Recombinant human SOX2 protein

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Sequence info

[{"sequence":"MEPAAGSSMEPSADWLATAAARGRVEEVRALLEAGALPNAPNSYGRRPIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPD","proteinLength":"Full Length","predictedMolecularWeight":"16.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":156,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P42771","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
False
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Specifications

Form

Lyophilized

Additional notes

Purity is greater than 95% by SDS-PAGE gel and HPLC analyses.

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General info

Function

The protein expressed by the gene CDKN2A acts as a negative regulator of normal cell proliferation by strongly interacting with CDK4 and CDK6. This interaction inhibits the ability of CDK4 and CDK6 to interact with cyclins D and to phosphorylate the retinoblastoma protein. This supplementary information is collated from multiple sources and compiled automatically.

Sequence similarities

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Post-translational modifications

Phosphorylation seems to increase interaction with CDK4.

Subcellular localisation

Nucleus

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Product protocols

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Target data

The protein expressed by the gene CDKN2A acts as a negative regulator of normal cell proliferation by strongly interacting with CDK4 and CDK6. This interaction inhibits the ability of CDK4 and CDK6 to interact with cyclins D and to phosphorylate the retinoblastoma protein. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CDKN2A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Asian Pacific journal of cancer prevention : APJCP 16:5115-8 PubMed26163652

2015

The p16INK4a Antibody Immobilization Method for Immonosensor Application.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yang,Xian-He Huang,Liang Sun

Asian Pacific journal of cancer prevention : APJCP 15:9375-8 PubMed25422227

2014

A piezoelectric immunosensor for early cervical cancer detection.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yang,Xianhe Huang,Liang Sun
View all publications
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Product promise

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