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Recombinant Human CDNF protein is a Human Fragment protein, in the 25 to 187 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS.

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Images

Mass Spectrometry - Recombinant Human CDNF protein (AB315437), expandable thumbnail
  • HPLC - Recombinant Human CDNF protein (AB315437), expandable thumbnail

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, MS

Biologically active

No

Mass spectrometry

LC-MS/MS

Amino acid sequence

Q G Q E A G G R P G A D C E V C K E F L N R F Y K S L I D R G V N F S L D T I E K E L I S F C L D T K G K E N R L C Y Y L G A T K D A A T K I L S E V T R P M S V H M P A M K I C E K L K K L D S Q I C E L K Y E K T L D L A S V D L R K M R V A E L K Q I L H S W G E E C R A C A E K T D Y V N L I Q E L A P K Y A A T H P K T E L

Reactivity data

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).

Alternative names

Recommended products

Recombinant Human CDNF protein is a Human Fragment protein, in the 25 to 187 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS.

Key facts

Purity

>=95% HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

HPLC, MS

Accession
Q49AH0-1
Animal free

Yes

Species

Human

Reconstitution

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Q G Q E A G G R P G A D C E V C K E F L N R F Y K S L I D R G V N F S L D T I E K E L I S F C L D T K G K E N R L C Y Y L G A T K D A A T K I L S E V T R P M S V H M P A M K I C E K L K K L D S Q I C E L K Y E K T L D L A S V D L R K M R V A E L K Q I L H S W G E E C R A C A E K T D Y V N L I Q E L A P K Y A A T H P K T E L

Accession

Q49AH0

Protein length

Fragment

Predicted molecular weight

18.57 kDa

Amino acids

25 to 187

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).

Sequence similarities

Belongs to the ARMET family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CDNF also known as Cerebral Dopamine Neurotrophic Factor is a protein that helps in maintaining the proper function of neurons. It has a mass of approximately 18 kDa. This protein is widely expressed in various tissues especially in the brain. CDNF maintains endoplasmic reticulum homeostasis and is involved in the stabilization of dopaminergic neurons. It does this by reducing stress in the endoplasmic reticulum and promoting cell survival mechanisms.

Biological function summary

The protein ensures the survival of neurons by modulating cellular stress pathways. CDNF is not known to be part of a larger protein complex. Instead its activity relies on its own structural configuration to influence stress responses and neuron protection. As a secreted protein it directly interacts with and influences cellular mechanisms within the extracellular space contributing to a healthy neuronal environment.

Pathways

CDNF interacts with at least oxidative stress response pathways and neuroprotection pathways. These pathways underpin its role in counteracting cellular stress related to neurodegeneration. For example CDNF shares a functional connection with proteins like MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) another neurotrophic factor involved in similar neuroprotective pathways enhancing neuronal survival and recovery from cellular stress.

Associated diseases and disorders

Neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease are major areas where CDNF shows relevance. In Parkinson's disease CDNF's neuroprotective role helps mitigate the loss of dopaminergic neurons. There is evidence linking CDNF with MANF which also exhibits protective roles in neuronal health. Both proteins aim to promote neuron survival amid progression of neurodegeneration associated with these diseases indicating their potential as therapeutic agents.

Product promise

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2 product images

  • Mass Spectrometry - Recombinant Human CDNF protein (ab315437), expandable thumbnail

    Mass Spectrometry - Recombinant Human CDNF protein (ab315437)

    Mass determination by ESI-TOF.

    Predicted MW is 18570 Da (+/- 10 Da by ESI-TOF). Observed MW is 18570 Da.

  • HPLC - Recombinant Human CDNF protein (ab315437), expandable thumbnail

    HPLC - Recombinant Human CDNF protein (ab315437)

    HPLC analysis of ab315437

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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